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InVet
versión On-line ISSN 1668-3498
Resumen
ACUNA, S y FUMUSO, E. Presence and distribution of steroid receptors and antigen of cell proliferation in immune cells in endometrium of mare. InVet [online]. 2013, vol.15, n.1, pp.29-36. ISSN 1668-3498.
Alpha estrogen receptors, progesterone receptors, and cell proliferation antigen were studied in endometrial immune cells of mares free of endometritis. Endometrial biopsies and blood samples were taken in healthy mares in four moments: at the start of the follicular phase , at 24 hours after estrus detection , at 24 hours post-ovulation and on day 7 post- ovulation. Biopsies were stained with hematoxylin-eosin or subjected to immunohistochemistry to determine the presence of RE±, RP or PCNA in mononuclear or polymorphonuclear cells. Statistically significant changes were detected at day 7 post-ovulation, compared to other moments. High levels of plasmatic progesterone were associated to a decrease in the total amount of immune cells and a reduction in the number of polymorphonuclear for estrogen receptor alpha. It is concluded that high plasma progesterone levels at 7 post-ovulation negatively modulated the number of immune cells and the number of polymorphonuclear immunelabeled for estrogen receptor alpha.
Palabras clave : Immune cells; Steroid receptors; Mares.