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InVet
versión On-line ISSN 1668-3498
Resumen
COLLA, C et al. Estimation of interferences in the determination of fructosamine of feline and canine serum. InVet [online]. 2014, vol.16, n.2, pp.63-68. ISSN 1668-3498.
The fructosamine serum concentration reflects the degree of glycemic control obtained during a preceding period. Usually, the presence of hemoglobin or bilirrubin in blood samples, could cause interferences in the laboratory analytical assays.Thus, we investigated the possible interferences on fructosamine determinations in serum from healthy cats and dogs. To testing and quantify the interference, we construct interferograms adding increased concentrations of hemoglobin or bilirrubin to serum pools of cats or dogs. We tested serum hemoglobin concentrations from 0.19 to 7.5 g/l in feline sera and 0.16 to 6.10 g/l in canine sera. The bilirrubin testing was done from 6 to 600 mg/l in both species sera. The results had shown a hemoglobin positive and additive interference in feline sera (≥1.60 g/l) and a negative interference in canine sera (≥1.20 g/l). In addition both species had shown positive and additive bilirrubin interferences (≥30 mg/l for feline sera and ≥150 mg/l for canine sera)
Palabras clave : Interferences; Fructosamine; Canine; Feline.