Abstract

ALBO, GN. et al. Development of an immunodiffusion technique for the detection of Sacbrood bee virus. InVet [online]. 2016, vol.18, n.2, pp.363-370. ISSN 1668-3498.

Beekeeping is one of the most important agricultural activities in Argentine.In recent years, there has been a decrease in the number of bees worldwide. The mortality of bees can be attributed to several factors, such as the interaction of environmental factors, wrong management of colonies or the action of various patognes, including viruses. Considering that in Argentina, only molecular diagnostic techniques are available for the detection of viruses, the objective of this work was to develop an immunodiffusion test that allows the detection of the sacbrood bee virus as a simpler and Economical alternative. To developed the immunodiffusion test we use an indigenous virus strain of sacbrood was used to inoculate a rabbit from which specific antibodies were obtained against this virus. Later were used to standardize the immuodiffusion technique. This technique could be very useful in the future to carry out a rapid survey of the sanitary conditions for SBV in hives of Argentine, because due to its low complexity it could be carried out in laboratories of low complexity throughout the country.

Keywords : Honey bees; Virus; Sacbrood; Immunodiffusion.