Resumen

GARBACCIO, S.G et al. Diagnóstico bacteriológico de tuberculosis bovina en bovinos reactores positivos a la prueba tuberculínica. RIA. Rev. investig. agropecu. [online]. 2018, vol.44, n.1, pp.69-75. ISSN 1669-2314.

Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), considered a zoonotic disease. In our country, the diagnosis is based on the application of the tuberculin skin test (TST). Conventional culture, from various biological samples, can complement the TST test results. Regarding this, it is possible to perform sampling and subsequent analysis of nasal discharge, milk and tissues. The latter allows the observation of macroscopic lesions, the anatomical location in addition to collect biological samples to be analyzed by bacteriology and histopathology. The aim of this study was to evaluate the presence of M. bovis in samples of nasal secretions, milk and tissues in cattle PAC-positive and characterize gross and microscopic lesions compatible with BTB. A total of 744 samples belonging to 572 cattle from dairy herds in the provinces of Córdoba, Santa Fe and Buenos Aires, were processed. All of them were, during this study, in stage of control of BTB showing a low prevalence (between 0.5 to 4%). About 12.4% (71/572) M. bovis isolations from cattle examined was obtained. These isolations were from tissue samples 36.5% (38/104), followed by milk 10.7% (23/214) and nasal secretions 3.9% (10/254). Bacteriology from milk samples and nasal secretions were of limited effectiveness. On the other hand, tissue analysis was more useful as complementary diagnostic, allowing confirmation of suspected cases of BTB.

Palabras clave : Mycobacterium bovis; Bacteriology; Tissues; Cattle.

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