Abstract

DI CIACCIO, L.S; FORTUNATO, R.H  and  SALVAT, A.E. Actividad antifúngica de especies del género Senna (Caesalpinoideae, Leguminosae) del norte de Argentina frente a Fusarium verticillioides. RIA. Rev. investig. agropecu. [online]. 2018, vol.44, n.1, pp.111-120. ISSN 1669-2314.

The genus Senna comprises about 360 species being 80% from the American continent. Several of them are known by their antimicrobial and pharmacological properties. In Argentina antecedents of antifungal activities were reported in studies of methanolic extracts from leaves of S. spectabilis against Fusarium graminearum. The aim of this work was to analyze the antifungal activity of 12 methanolic extracts of different organs of 10 1Instituto Nacional de Tecnología Agropecuaria (INTA), Centro de Investigación en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de Patobiología. CONICET. Nicolas Repetto y de los Reseros s/n, Hurlingham (1686), prov. de Buenos Aires, Argentina. species of the genus Senna collected from northern Argentina against Fusarium verticillioides, causal agent of maize ear rot. At first all the extracts were evaluated to know their antifungal capacity with a colorimetric test. Those which showed inhibitory activity were analyzed by contact bioautography, Evans blue and Aniline blue staining, mycelial radial growth test and also their ability to inhibit fumonisins production. Fruit extract of S. spectabilis showed antifungal activity at a Minimun Inhibitory Concentration of 99.9 μg ml-1. Likewise, in the contact bioautography an inhibition zone was evident, while in Evans blue and aniline blue staining, some alterations in the mycelial hyphae were visualized. In the mycelial radial growth test, a significant reduction was observed, and in the fumonisins production the extracts from fruits showed inhibition of 48.3% while in flowers the percentage was higher (86.3%). Finally, it was determined that S. spectabilis has antifungal activity against F. verticillioides, giving a new record of bioactivity to this plant species.

Keywords : Native plants; Antifungal activity; Extracts; Fumonisins.

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