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Ciencia del suelo
versión On-line ISSN 1850-2067
Resumen
DIOVISALVI, Natalia Veronica et al. Methods to determine nitrogen in sunflower grains. Cienc. suelo [online]. 2021, vol.39, n.2, pp.16-31. ISSN 1850-2067.
In sunflower, grain oil concentration (OG) determines oil industrial yield and protein concentration (PG) determines the protein of by-products as pellets and meals (PM). There are various methodologies to quantify total grain nitrogen concentration (NG): a) Kjeldahl (wet digestion); b) Dumas (dry combustion); c) NIRS (analysis of the spectrum of the light reflected by the sample). NIRS represents a promissory method for determining NG because it is simple and fast. The objective of this work was to compare the methods of NIRS, Kjeldahl and Dumas to quantify NG in sunflower grains and to adjust models to estimate PM from NG. Eighty-four samples of sunflower grains were selected covering a wide range of NG (from 1.75 to 3.80% taking Dumas reference) from a net of experiments conducted in southeastern Buenos Aires Province (2013-2014 and 2014-2015). NIRS was the most precise method for the determination of NG and presented a greater degree of agreement with Dumas than Kjeldahl. PM was satisfactorily estimated with NG. Therefore, NIRS could be a better alternative to the other techniques because its low cost, quickness, low toxicity, and adaptability to determine other components of the grain simultaneously with the determination of NG.
Palabras clave : Dumas; Kjeldahl; NIRS; protein.