Ciencia, docencia y tecnología
On-line version ISSN 1851-1716
GALUSSI, Alberto A et al. Identification of Medicago sativa L. cultivars by its biochemical characteristics in seeds and seedlings. Cienc. docencia tecnol. [online]. 2011, n.43, pp. 201-216. ISSN 1851-1716.
Since alfalfa cultivar seeds are morphologically similar, seed and seedling protein analysis would be adequate to differentiate both cultivars. The purpose of this study was to characterize alfalfa cultivars from two different harvests according to seed protein level determined through SDS PAGE and A PAGE, and in protrusion and cotyledonar state through dehidrogenase alcohol isoenzyme. The adequate sample size for electrophoresis was 0.5g of seeds in 5.00ml of extraction buffer and the appropriate sowing volume was 10μl. In both SDS PAGE and A PAGE methods, protein fractioning was carried out though it was not possible to differentiate among cultivars; the slight differences found were not repeated in samples from different harvest years. The ADH system showed differences among cultivars getting a higher differentiation at the protrusion state.
Keywords : Botany; Alfalfa; Electrophoresis; Proteins; Isoenzymes.