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vol.44 issue3-4Comparison of culture conditions for isolation and count of soil yeasts from Nothofagus spp. (Fagaceae) native forest of Argentinean PatagoniaThe family Geoglossaceae s. str. (Helotiales) from Tucumán, Argentina author indexsubject indexarticles search
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Boletín de la Sociedad Argentina de Botánica

On-line version ISSN 1851-2372

Abstract

ULLOA, José; LIBKIND, Diego; FONTENLA, Sonia  and  VAN BROOCK, María. Fermenting yeasts isolated from Cyttaria hariotii (Fungi) in the Andean Patagonic Forest. Bol. Soc. Argent. Bot. [online]. 2009, vol.44, n.3-4, pp.239-248. ISSN 1851-2372.

Yeasts have been associated with mankind from early ages. Among these, fermentative yeasts played the most relevant role, due to its importance in the food industry. The biotechnological implications of these yeasts have been extensively studied. However there is a lack of information about its distribution in nature and ecology. In this study samples of Cyttaria hariotii, ascomycete fungus parasite of trees of the genus Nothofagus, were collected in Nahuel Huapi National Park (Northwestern Patagonia, Argentina). 72 isolates were obtained using a selective culture medium with 8% ethanol. Identification of the isolates was based on morphological and physiological tests, and then sorted into five groups. Three groups were assigned to the genera Saccharomyces, Pichia and Kloeckera respectively. The Saccharomyces group was the prevailing one, and was sorted into three subgroups. Two of them were related to S. bayanus and /or S. uvarum. All the isolates were psychrotolerant and the maximum growth temperature ranged between 35 and 37 _C. This work contributes to the knowledge of the biodiversity of yeasts in the region and represents the first massive isolation of Saccharomyces yeasts in natural environments of Patagonia.

Keywords : Fermenting yeasts; Cyttaria hariotii; Yeasts biodiversity; Yeasts physiology; Psychrotolerant; Andean Patagonic Forest.

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