Abstract

FRADKIN, Maia; GREIZERSTEIN, Eduardo J; FERRARI, María R  and  POGGIO, Lidia. Genome characterization of a synthetic Triticum x Thinopyrum (Poaceae) amphiploid usingin situ hybridization. Bol. Soc. Argent. Bot. [online]. 2014, vol.49, n.4, pp.541-546. ISSN 1851-2372.

"Trigopiros" derive from crosses between different species of Triticum L. and Thinopyrum Löve. These synthetic amphiploids are designed with the aim to obtain cereals similar to wheat but which are perennial, resistant to diseases and to the salinity of the soils. Moreover, they allow the transfer of the agronomic attributes of Thinopyrum to wheat. "Trigopiro" Don Noé INTA, which is currently grown in Argentina, presents valuable agronomic traits as well as a high content of seed proteins. In the present work, the use of classical cytogenetic techniques allowed us to confirm that the chromosome number of "Trigopiro" Don Noé is 2n=56. The application of in situ hybridization (FISH-GISH) allowed us to postulate its genomic composition for the first time. This artificial hybrid has 14 chromosomes from genome J of Thinopyrum and 2 chromosomes pairs with putative translocations between Triticum and Thinopyrum. The rest of chromosomes belong to A, B and D genomes of Triticum.

Keywords : Trigopiro; FISH; GISH.

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