Resumen

ARANGO et al. Histological Characteristics Of Skin Culture In Vitro. Rev. argent. dermatol. [online]. 2009, vol.90, n.4, pp.00-00. ISSN 1851-300X.

In vitro skin culture have been used in the regeneration of skin wound (chronic ulcers and burns), and for genodermatosis treatment. In our country there is a high patient number with chronic ulcers and 2319 burned in a period of 10 years. Conventional treatment generates long hospitalization stays and high costs. We established culture conditions of keratinocytes and fibroblasts expansion, to generate a cutaneous substitute in order to offer other treatment options. Skin cells were obtained from organs donors and surgical surpluses procedures. Major success was achieved in primary cultures obtained from 40-year-old younger donors samples (65%), compared with older donors (33%). In one cutaneous substitute produced with these cells, was demonstrated that keratinocytes and fibroblasts, presented functional, structural and morphologic characteristics similar to normal skin. Cutaneous substitute besides preserve normal skin functional and structural characteristic, compared with other similar imported products, our cutaneous substitute, showed many advantages in terms of costs, manipulation and stability.

Palabras clave : Skin substitute; Keratinocytes; Fibroblasts; Cell culture; Regenerative medicine.

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