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vol.83 issue1-2Seedcane production in Registered Nurseries obtained through micropropagation and hot water treatment of three sugarcane cultivars author indexsubject indexarticles search
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Revista industrial y agrícola de Tucumán

On-line version ISSN 1851-3018

Abstract

DIGONZELLI, Patricia A. et al. Potential germination dynamics of micropropagated and hot-water-treated seedcane of three sugarcane cultivars. Rev. ind. agríc. Tucumán [online]. 2006, vol.83, n.1-2, pp.1-7. ISSN 1851-3018.

The potential germination dynamics (germination under optimal humidity and temperature conditions) of healthy seedcane of three cultivars (LCP 85-384, CP 65-357 and CP 48-103) at three different ages (7, 10 and 12 months) were compared, by studying one-joint billets of seedcane obtained through micropropagation and hot water treatment (inmersion into water heated at 50ºC, for 2 h). This micropropagated and hot-water-treated seedcane was free from Ratoon Stunting Disease or RSD (Leifsonia xyli subsp. xyli) and leaf scald disease (Xanthomonas albilineans). The billets were kept on washed and sterilized sand trays in a growing chamber, at a temperature of 26 ± 2ºC, under constant light and humidity control. A completely randomized factorial experimental design (3x3x2), with two replications, was used. The factors considered were: age (three levels), cultivar (three levels) and seedcane origin (two levels). The emerged buds (visible shoots) were counted every day until germination became stable. The potential germination dynamics of the studied material adjusted to a single exponential model, symmetrical sigmoidal function, with R2 ≥ 0,97. This model was used to obtain the deduced variables t50 and t90, (days to reach 50% and 90% of total emergence), maximum germination percentage and average and maximum potential germination rates. Micropropagated seedcane presented a faster shoot development, over a shorter span, than hot-water-treated seedcane. The final percentage varied from 80% to 100%, regardless of seedcane origin.

Keywords : High quality seedcane; Micropropagation; Hot water treatment; Emergence.

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