Resumo

POLLO, Favio E; SALAS, Nancy E; MANCINI, Miguel A  e  MARTINO, Adolfo L. Comparative studies of the frequency of micronuclei and nuclear abnormalities in erythrocytes of three species fishes. Acta toxicol. argent. [online]. 2012, vol.20, n.2, pp.62-67. ISSN 1851-3743.

Aquatic ecosystems near urban areas suffer a steady deterioration caused by physical and chemical factors related to human activity, representing a danger to organisms that inhabit them, especially the fsh. These effects may be biomonitored by widely used techniques, such as the Micronucleus Test (Mn) and Nuclear Abnormalities (NA), among others. The aim of this study was to verify and compare the frequency of micronuclei and nuclear abnormalities in three fsh species collected in an urban lake, Cyprinus carpio, Astyanax eigenmanniorum and Cheirodon interruptus. After the capture and anesthesia of the fsh, weight and length were registered, and a blood sample was obtained from each individual. The samples were fxed and stained with 10% Giemsa, subsequently they were analyzed with the microscope (1000X) and photographed with a digital camera. The body con-dition index (K) was calculated for each fsh caught. A correlation analysis Spearman rank (rs) between frequency of Mn and NA vs. K was performed for each species. The Kruskal-Wallis test was applied to assess differences in Mn and NA between species. The results showed no statistically signifcant correlation (P> 0.05) between the frequencies of Mn and NA vs K. The frequencies of Mn between different species yielded no signifcant differences (P> 0.05), whereas NA (P <0.05) did. A. eigenmanniorum was the most sensitive species. NA preliminary differences between the species used, convert A. eigenmanniorum in a sort of "sentinel specie" and a possible biomonitor of xenobiotic agents.

Palavras-chave : Micronuclei; Nuclear abnormalities; Fish; Xenobiotics.

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