Phyton (Buenos Aires)
versión On-line ISSN 1851-5657
GARCIA-ZAMBRANO, Eduardo A et al. Clasificación y estimación de la diversidad genética de Nopal Opuntia spp en base a descriptores fenotípicos y marcadores genético molecularesClassification and estimation of genetic diversity in Nopal Opuntia spp based on phenotypic descriptors and genetic-molecular markers. Phyton (B. Aires) [online]. 2006, vol.75, pp. 125-135. ISSN 1851-5657.
The objective of this investigation was to develop and apply molecular markers (type RAPD) for estimating the genetic diversity of 100 Opuntia accessions from the Germoplasma Bank of the FAUANL. The molecular data were evaluated by multivariate analysis using the methods of Ward and UPGMA. These results were compared with phenotypic data taken from the same accessions, already evaluated by the above mentioned methods. The protocol was modified for the extraction of the Opuntia DNA to reduce the quantity of polysaccharides and their influence in its quality. Later on, a protocol was developed for the generation of molecular markers of the type RAPD for Opuntia accessions. This allowed to combine the accessions by the similarity degree of the molecular markers, in agreement with phenotype data.
Palabras clave : Molecular markers; RAPD; Accesion; PCR.