Phyton (Buenos Aires)
versión On-line ISSN 1851-5657
GIMENEZ, C; DE GARCIA, E y HADDAD, O. Genetic and resistance stability to Black Sigatoka disease during micropopagation of Musa CIEN BTA-03 somaclonal variant. Phyton (B. Aires) [online]. 2008, vol.77, pp. 65-79. ISSN 1851-5657.
Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with 10 different primer combinations. Non-polymorphic RAPD bands were found in this assay. Additionally, six plants of CIEN BTA-03 produced in the fifth cycle of micropropagation and the same number of plants from cultivars Pisang Mas, Williams and Yagambi Km5, were tested in the field to evaluate their response to Black Sigatoka disease. The clonally micropropagated CIEN BTA-03 plants preserved the Black Sigatoka resistance phenotype.
Palabras llave : Micropropagation; Genetic stability; RAPD; Disease resistance; Youngest leaf spotted (YLS).