Phyton (Buenos Aires)
On-line version ISSN 1851-5657
CERNA¹, E et al. Assessment of protein quantification methods in Tetranychus urticae, as a potential tool for resistance detection to pesticides. Phyton (B. Aires) [online]. 2010, vol.79, n.2, pp. 147-152. ISSN 1851-5657.
Protein assays were conducted on Tetranychus urticae Koch, as potential resistance detection tools to plaguicides. This is a phytophagous mite that feeds on a large variety of plants. Experiments were carried out using a pesticide susceptible and three field crop strains of T. urticae. Protein was measured by colorimetric assays, using Kit-II from Bio-Rad, with bovine serum albumin (BSA) as standard. Homogenates were prepared using 10, 30, 50, 100, 300, 500 and 800 mites, with 30 replicates each. Linearity was obtained for the standard curve of the different methods, and r2 values ranged from 0.877 to 0.985. The Bradford method modified by Brogdon gave the highest protein values for homogenates of 10, 30 and 50 mites. The susceptible laboratory strain showed 0.2148 μg protein per μg weight of mite; values showed by the field crop strains were 0.2059, 0.1703 and 0.1550 for the strawberry, Royalty rose and Rafaela rose mites, respectively. Adult females of T. urticae showed a higher amount of protein when they were 8 days old.
Keywords : Two-spotted spider mite; Linearity; Absorbance; Colorimetry; Protein.