Abstract

GRENON, Sandra L; MARTINEZ, Monica E  and  VON SPECHT, Martha H. Epidemiology of Pneumococcal Surface Protein a (PspA) IN Streptococcus pneumoniae Causing Invasive Infections in a Paediatric Hospital in Argentina. Rev. cienc. tecnol. [online]. 2016, n.25, pp.28-33. ISSN 1851-7587.

It has been emphasized Pneumococcal Surface Protein A (PspA) as a main component for developing a pneumococcal vaccine with the potential to offer a broad protection unrelated to serotype. The aim of this work was to determine the frequency and distribution of PspA families 1 and 2 of Streptococcus pneumoniae causing invasive pediatric infections. We worked with 91 isolates from the period 2005-2012. Antibiotic sensitivity was studied according to the Clinical and Laboratory Standards Institute, serotyping by Quellung technique, and the presence of PspA variant gen was determined by polymerase chain reaction. From the studied isolates, 48 (52.7%) were identified as family 1, 28 (30.8%) as family 2 and in 6 (6.6%) none of the desired amplicon was detected. Expression of both families was found in 9 isolates (10%). Family 2 frequencies were higher than expected among children <2 years and in cases of meningitis (p> 0.05). Sixteen different serotypes were detected; serotype 1 was related to family 1 and serotype 7F to family 2. Serotype 14 was associated with both families. No difference between the distribution of families and the results of minimum inhibitory concentration of penicillin and cefotaxime was detected. Our findings showed that the development of a PspA antigens based vaccine, containing both families 1 and 2 would be effective in the region.

Keywords : Streptococcus pneumonia; PspA; Invasive disease; Pediatrics.

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