Abstract

PONZINIBBIO, MV; CRUDELI, C; PERAL GARCIA, P  and  SEOANE, A. Cytogenetic and cytomolecular delayed damage induced in human fibroblasts by low X-ray doses. BAG, J. basic appl. genet. [online]. 2008, vol.19, n.1, pp.35-41. ISSN 1852-6233.

During the last decade several reports have provided evidence on the non-targeted effects of ionizing radiation. In this study, we have analyzed the delayed DNA damage induced by ionizing radiation in MRC-5 Human fibroblasts cultured in vitro. MRC-5 cells were irradiated with 10 and 50 milligrays (mGy) and analyzed immediately after exposure, and at passages 6 and 12 post-radiation by means of comet assay, micronucleus analysis and γ-H2AX foci formation. Cytogenetic and cytomolecular damage was analysed at every sample point comparing irradiated and control cells. No significant damage differences were found among samples from all passages of treatments. Our results suggest that even at low doses, ionizing radiation could induce delayed damage. Comet assay, micronucleus analysis and γ-H2AX focus analysis showed to be useful techniques to detect DNA damage and might be also sensitive for the study of delayed events as genomic instability.

Keywords : Ionizing radiation; Low radiation dose; DNA damage.

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