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BAG. Journal of basic and applied genetics

versión On-line ISSN 1852-6233

Resumen

MARRUBE, G et al. Análisis del efecto de polimorfismos de un solo nucleótido del Gen Fasn en novillos. BAG, J. basic appl. genet. [online]. 2010, vol.21, n.1, pp.43-48. ISSN 1852-6233.

Fatty acid synthase (FASN) is an enzyme that catalyzes the synthesis of fatty acids in the cell and has a key role in lipid metabolism. For the purpose of analyzing the effect of two Single Nucleotide Polymorphisms (SNPs) in the FASN gene (G>C in exon 1 and A>G in exon 34) the following productive traits were analyzed: Final Live Weight, Live Weight Daily Gain, Fat Thickness Monthly Gain, Kidney Fat, Kidney Fat Percentage, Carcass Yield, Intramuscular Fat Percentage, in Aberdeen Angus (n = 20) and Brangus (n = 60) steers. Mismatch PCR techniques were developed to determine both SNPs genotypes. Phenotypic data were analysed with a model that included the fi xed effects of SNP genotypes and genetic group. For the SNP in exon 1 signifi cant differences between genotypes (p<0,05) were found in Kidney Fat and Kidney Fat Percentage, while among genetic groups differences were found in every trait (p<0,05). According to the results obtained, the C allele from this SNP is associated with less Kidney Fat and lower Kidney Fat Percentage. Due to the absence of homozygous AA animals and the low frequency of heterozygous animals, statistical analyses for polymorphism in exón 34 could not be performed.

Palabras clave : Beef cattle; Meat quality; FASN gene; SNPs.

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