Abstract

BRUNO, Pablo Alexis et al. Osseointegration of cryopreserved allograft associated with platelet-rich plasma, in infected bone defects: Experimental study. Rev. Asoc. Argent. Ortop. Traumatol. [online]. 2013, vol.78, n.2. ISSN 1852-7434.

Blackground: The objective of this experimental work was to determine whether the addition of platelet-rich plasma influences the osseointegraton of cryopreserved allograft with antibiotics in infected fractures with bone loss, in long bones of rabbits. Methods: Four groups of 7 rabbits with generated infected fracture of the right femur. Milled allograft with local antibiotic was placed in the four groups; groups II and IV also received platelet-rich plasma. Rabbits in groups I and II were sacrificed at 30 days, and those of groups III and IV at 90 days. Histological and CT evaluation was performed. Results: Nine rabbits died during the study period. There were no statistically significant differences between groups. In group IV with platelet-rich plasma, bone formation was observed in three of the four samples, with noticeable areas of coupling between the receiver and the grafted tissue with consolidation in CT. Conclusions: In our study, there was no correlation between the histological and tomographic findings. Although we found no statistically significant differences, the addition of platelet-rich plasma stimulated the generation of new tissue, and also decreased reabsorption of the grafted tissue according to the pathologic analysis. Besides increased bone formation and consolidation was detected in group IV (75%) treated with platelet-rich plasma, compared to group III (25%) without platelet-rich plasma bone allograft.

Keywords : Infected fractures; Allograft bone; Platelet-rich plasma.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )