Abstract

CAGNOLO, Susana R; CARRANZA, Franco; TRIMARCHI, Laura  and  BERTOLOTTI, María A. New findings of Heterorhabditis bacteriophora and Steinernema rarum (Nematoda: Heterorhabditidae, Steinernematidae) in Córdoba, Argentina. Rev. Mus. Argent. Cienc. Nat. [online]. 2016, vol.18, n.2, pp.191-199. ISSN 1853-0400.

A total of 152 soil samples collected from gardens of private properties at Córdoba city, Argentina, were evaluated for the presence of entomopathogenic nematodes (EPNs) and samples were analyzed for granulometry, relative humidity and pH. The Galleria bait method was used to isolate EPNs. The nematodes were characterized using both classic (morphometric characters) and molecular methods (analysis of internal transcribed spacer (ITS) and D2/D3 sequences of 28S genes). Phylogenetic analyses were performed to prove the identity of the studied nematodes. Recovery frequency of EPNs was 10.53%. Isolates belonging to the genera Heterorhabditis Poinar and Steinernema Travassos were detected. Two isolates were identified as Steinernema rarum (Doucet) Mamiya and another four as Heterorhabditis bacteriophora Poinar. No significant differences were found in edaphic characteristics among sites with presence or absence of EPNs. The known geographical distribution of S. rarum and H. bacteriophora is expanded, and the diversity of populations of the nematodes suitable to be used against insect pests of urban areas is increased.

Keywords : Entomopathogenic nematodes; Detection; Edaphic characteristics; Urban zone.

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