Abstract

ESPOSITO, María Andrea; MARTIN, Eugenia; CRAVERO, Vanina P  and  COINTRY, Enrique L. Use of morphological, biochemical and SRAP molecular markers to differentiate varieties of Cynara cardunculus L. (Asteraceae). Rev. Fac. Cienc. Agrar., Univ. Nac. Cuyo [online]. 2011, vol.43, n.2, pp.35-45. ISSN 1853-8665.

In the Cynara cardunculus L. primary complex we can find a globe artichoke: Cynara cardunculus var. scolymus and other botanical varieties such as C. cardunculus var. altilis (cultivated cardoon) and C. cardunculus var. sylvestris (wild artichoke). Traditionally, they varieties were grouped according to morphological and biochemical traits and more recently, based on molecular markers. The aim of the present paper was to compare the use of morphological, biochemical and molecular markers to distinguish botanical varieties of Cynara cardunculus. Three accessions of var. scolymus, two accessions of var. altilis and two accessions of var. sylvestris were used. Different morphological variables, electrophoresis seed protein patterns by SDS-PAGE and SRAP markers were evaluated. The three kinds of markers grouped all materials in two clusters: one of them included the globe artichoke and the other the cardoons. SDS-PAGE is a valid technique for botanical varieties identification but the morphological and molecular data were more effective at discriminating botanical varieties and accessions within the scolymus group.

Keywords : Cynara cardunculus; Botanical varieties; Morphological markers; Seed protein; SRAP.

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