Revista argentina de cirugía
versión ISSN 2250-639X
DIP, F et al. Utilización de verde de indocianina fluorescente intraoperatorio en cirugía biliar: Factibilidad del método: experiencia inicial. Rev. argent. cir. [online]. 2011, vol.100, pp. 19-22. ISSN 2250-639X.
Introduction: Indocyanine green dye is hydrophilic due to albumin binding. The dye has fluorescent properties when excited with light of 780 nm (near infrared) and captured with a filter wavelength of 830 nm. It is excreted exclusively via the bile, which would make it suitable as cholangiographic method. Objective: To evaluate the feasibility of using indocyanine green as fluorescent dye for visualization of the liver and bile ducts by near-infrared stimulation. Application: Experimental Surgery Center University Hospital de Clínicas José de San Martín. Design: Prospective, descriptive, experimental. Population: 10 wistar rats of 350 grams laboratory. Method: Anesthesia with ketamine intraperitoneal xylasina. Then performed an inguinotomy, iliac vein dissec-tion intravenous injection of indocyanine green contrast and then we proceeded with a laparotomy. During surgery we evaluated the anatomy of the liver and bile duct field exposure alternating with white xenon light and infrared. Results: In evaluating the operative field with xenon light was observed the liver and duodenum of the rats but could not be clearly identified in any case, the bile duct. When we used optical and infrared vision camera with 830 nm filter were visualized in all cases the bile duct in its entirety and duodenum extrahepatic fluorescent blue color. The hepatic vascular structures of the hilium, both hepatic artery and portal vein, were visualized as tubular structures in black color. Conclusion: Exposure of indocyanine green to infrared light of 780 nm generates a blue fluorescent projection insensitive to human vision but sensitive to 830 nanometer filter. When injected intravenously and with its exclusively biliary excretion, allows viewing on a fluorescent hepatobiliary anatomy.
Palabras llave : Biliary duct; Research surgery.