versión impresa ISSN 0002-7014
Ameghiniana vol.47 no.1 Buenos Aires ene./mar. 2010
Multiple dinosaur egg-shell occurrence in an Upper Cretaceous nesting site from Patagonia
Rodolfo A. Coria1,2, Leonardo Salgado1,3 and Luis M. Chiappe4
2Subsecretaría de Cultura de Neuquén - Museo Carmen Funes, Av. Córdoba 55, 8318 Plaza Huincul, Neuquén, Argentina. firstname.lastname@example.org
3Inibioma-Museo de Geología y Paleontología, Universidad Nacional del Comahue, Buenos Aires 1400, 8300 Neuquén, Argentina. email@example.com
4Dinosaur Institute, Los Angeles County Museum of Natural History, Los Angeles, California. firstname.lastname@example.org
The discovery of hundreds of megaloolithid-type egg-clutches (some including embryos of an indeterminate species of titanosaur sauropods) in several stratigraphical levels of the Late Cretaceous nesting site of Auca Mahuevo (Chiappe et al., 2005) unveiled important aspects of the reproductive behavior of sauropod dinosaurs and stimulated further work at other Patagonian dinosaur egg sites. In November of 2003, a join expedition of the National University of Comahue (Neuquén), the Museo Carmen Funes (Plaza Huincul) and the Museo de Lamarque (Lamarque) conducted fieldwork in several Late Cretaceous localities of Bajo Santa Rosa (center-north Río Negro Province, Argentina) bearing the remains of dinosaur eggs and other terrestrial vertebrates (figure 1).
Figure 1. 1, General location map of the fossil site Berthe IV. 2, location of the fossil site Berthe IV within the area of the Bajo Santa Rosa (the spot is marked by the star). 3, simplified geologic section of the site showing the stratigraphical position of the egg-bearing layer 4 (modified from Salgado et al., 2007) / 1, Mapa de localización general del sitio fosilifero Berthe IV. 2, localizacion del sitio fosilífero Berthe IV dentro del área del Bajo Santa Rosa (sitio indicado por la estrella). 3, perfil geológico simplificado del sitio indicando la posición estratigráfica de la capa portadora de huevos 4 (modificado de Salgado et al., 2007).
The main focus of this expedition was to assess the diversity of dinosaur eggs, to fine-tune the stratigraphy of the egg-bearing layers, and to document the spatial distribution of the egg-clutches. The overall results of this research were reported else-where (Salgado et al., 2007). In this contribution, we make focus in one of the fossil localities worked, Berthe IV, where the association of egg clutches likely belonging to different dinosaur species was detected (figure 1.2).
Collected eggshells were observed with a binocular loupe ''Stemi SV6 Zeiss'', at magnifications of x1.0 and x3.2 (for macrocharacters). Microcharacters were observed in transverse thin section using a polarizing microscope (''Zeiss Axioplan'') at magnifications of x0.4 and x10. The eggshells were photographed using this polarizing microscope equipped with a digital camera. Prior to analysis, the eggshells were submitted to a process of cleaning using ultrasound (see Salgado et al., 2007 for further methodological details).
Institutional abbreviations. MML-Pv, Museo Municipal de Lamarque, Vertebrate Paleontology, Río Negro, Argentina.
The central region of the Río Negro province is characterized by a series of shallow continental basins originated by both structural and eolian processes (González Diaz and Malagnino, 1984). The Allen Formation (middle Campanian-lower Maastrichtian) outcrops along the edges of these shallow basins and it contains the province's main egg-bearing deposits. The Allen Formation consists of a thick succession of sandstones and mudstones, which upper section is intercalated by carbonate and evaporitic deposits. Leanza and Hugo (2001) proposed the Allen Formation as a continental lithostratigraphic unit with a lower member characterized by fluvial deposits of moderate energy, a middle member of lacustrine and low-energy fluvial sediments, and an upper member containing shallow lacustrine deposits with evaporitic facies (Andreis et al., 1974). The egg-bearing layers described here are located within a lower subunit of the middle member, which comprises fine-grained sandy deposits with subordinate muddy layers and two thin evaporitic levels (figure 1.3). On the basis of sedimentological and lithofacies characters, this lower subunit, which yields vertebrate fossils, is interpreted as reflecting brackish lagoonal and supratidal environments, associated with aeolian sands (dunes) and deposits of ephemeral rivers. Abundant rhizoturbation, termite structures, and caliches in the egg-bearing deposits indicate the presence of palaeosols, which presumably developed in a supratidal environment in close proximity to the coast (Salgado et al., 2007).
Description of the egg-clutches and eggshells
We report the discovery of two different types of egg-clutches found next to one another in the same stratigraphic horizon (figure 2). The specifics of each of these egg-clutches and the morphology of the eggshell are described below.
Figure 2. Picture depicting the relative position of the clutches described here. The black line shows the egg-bearing horizon. 1, Megaloolithidae type egg-clutch MML-Pv 41. 2, Faveoloolithidae type egg-clutch / imagen indicando la posición relativa de las acumulaciones de huevos aquí descriptas. La línea negra muestra el horizonte portador de los huevos. 1, acumulación de huevos tipo Megaloolithidae MML-Pv 41. 2, acumulación de huevos tipo Faveoloolithidae.
Type I—Faveloolithid. This clutch is formed by three collapsed eggs, which are possibly the remnants of a larger clutch (the egg-clutch is located at the edge of a hill) (figure 2.2). The eggs are spherical and 18-21 cm in diameter. The three preserved eggs are aligned, although given the fact that these eggs are possibly remnants of a larger clutch, it is impossible to determine if the egg-clutch had any particular spatial arrangement. As in the case of the adjacent megaloolithid egg-clutch, the eggs of the faveloolithid clutch show no indication of being hatched and no evidence of embryos.
The outer surface is sculptured by nodular, sub-polygonal to rounded, non-coalescent structures. The diameter of the nodules varies between 0.32 and 0.80 mm, but more frequently within 0.48 and 0.80 mm (figure 3.1). The thickness of the eggshell varies from 4 to 6 mm (figure 3.2). The pore apertures are sub-circular with an average diameter of 0.16 mm; the pore system is multicanaliculate. On the inner surface, the mammillary structures are irregular, with diameters varying between 0.32 and 0.80 mm, and coalescent in groups of two, three or more. Typically, the shell units are forked with an inner mean diameter of 0.32 mm and outer mean diameter of 0.80 mm. The height of the shell units varies from 4.0 to 4.8 mm. The accretionary lines are arched.
Figure 3. Outer surface and radial thin sections of eggshells of Faveoloolithidae type (1, 2) and Megaloolithidae type (3, 4) / superficies externas y cortes delgados radiales de las cáscaras de los huevos tipo Faveoloolithidae (1, 2) y Megaloolithidae (3, 4).
Type II—Megaloolithid. This clutch is roughly 1 meter in diameter and contains 14 sub-spherical eggs, averaging 12-14 cm in diameter (MML-Pv 41, figure 2.1). The eggs do not exhibit any distinguish-able spatial arrangement. Given that they are stacked one on top of the other (2-3 layers), the eggs were probably laid in a depression, although unlike some nests from Auca Mahuevo (Chiappe et al., 2004), there is no direct evidence of the nest architecture. The eggs do not appear hatched but examination of a few eggs by mechanical removal of eggshell has failed to reveal any embryonic remains.
The ornamentation of the outer surface is composed of rounded nodules (ranging in diameter from 0.32 to 0.80 mm), a design characteristic of the com-pactituberculate surface morphology (figure 3.3). The eggshells are thin, with a mean thickness of 1.23 mm (figure 3.4). Pore openings are sub-circular, with diameters averaging 0.16 mm (the pore system is tubocanaliculate). The microstructure of the shell units have a fan-like outline, with a outer section ranging in width between 0.64 to 0.8 mm and a inner width of 0.32 mm. The height of the eggshell unit varies from 0.8 to 1.12 mm. The accretionary lines are arched.
The dinosaur nesting sites of the Allen Formation are widely distributed in the Río Negro Province. Although less spectacular than Auca Mahuevo, the Río Negro nesting sites preserve a greater diversity of eggshell types (Salgado et al., 2007): (1) Megaloolithus patagonicus, the same type of eggs found at Auca Mahuevo (Chiappe et al., 2005), (2) a second variety of megaloolithid-type egg, (3) fave-oloolithid eggs, and (4) elongatoolithid eggs (Salgado et al., 2007).
A review of the literature shows that associations of clutches composed of different types of eggs in the same nesting horizon are extremely rare—multispe-cific associations of dinosaur eggs appear to be largely based on weathered eggshells (e.g., Vianey-Liaud and Crochet, 1993). At Bajo Santa Rosa, not only megaloolithid eggshells are found in association with favoloolithid eggshells but two clutches of different types of eggs have been discovered along side. Although time-averaging—the fact that these two egg-clutches could have been laid in a time span undetectable within the rock record—cannot be entirely ruled out, this evidence suggests that two different dinosaur species nested in proximity to one another. Egg discoveries at Bajo Santa Rosa also provide evidence in support of site fidelity, although this behav-ior is only evidenced by the multiple occurrences of the same types of eggshells (megaloolithid and faveloolithid) (Salgado et al., 2007).
The authors thank to all the crew members that carried on the field works at Bajo Santa Rosa. We also thank W. Evans for editing the manuscript, and the Municipalidad de Plaza Huincul, the Subsecretaría de Estado de Cultura de Neuquén, COPELCO Cooperativa Ltda. and the Dinosaur Institute of the Natural History Museum of Los Angeles County for their institucional support. Drs. C. Magalhaes Ribeiro and T. Manera reviewed the manuscript and their comments are greately appreciated. This contribution is part of the Proceedings of the Third International Symposium on Dinosaur Eggs, Babies, and Developmental Biology, held in Plaza Huincul, Neuquén, Patagonia, Argentina, April 13-15, 2006. Research supported by a NGS Grant 7396-03, and CONICET, PIP 6455 and PICT 357 to L.S.
1. Andreis, R.A., Iñiguez, A.M., Rodríguez, Lluch, J.J. and Sabio, D.A. 1974. Estudio sedimentológico de las formaciones del Cretácico Superior del área del Lago Pellegrini (Prov. de Río Negro, Rep. Argentina). Revista de la Asociación Geológica Argentina 29: 85-104. [ Links ]
2. Chiappe, L.M., Schmitt, J.G., Jackson, F. D., Garrido, A., Dingus, L. and Grellet-Tinner, G. 2004. Nest structure for sauropods: sedimentary criteria for recognition of dinosaur nesting traces. Palaios 19: 89-95. [ Links ]
3. Chiappe, L.M., Jackson, F.D., Coria, R.A., and Dingus, L. 2005. Nesting titanosaurids from Auca Mahuevo and adjacent sites (Late Cretaceous; Patagonia, Argentina): Understanding sauropod reproductive behavior and embryonic development. In: J. Wilson and K. Curry-Rogers (eds.), Sauropod Paleobiology, University of California Press, Berkeley, pp. 285-302. [ Links ]
4. González Díaz, E.F. and Malagnino, E.C. 1984. Geomorfología de la Provincia de Río Negro. 9º Congreso Geológico Argentino (San Carlos de Bariloche), Publicación Especial, Actas: 1-159. [ Links ]
5. Leanza, H.A. and Hugo, C.A. 2001. Cretaceous red beds from southern Neuquén Basis (Argentina): age, distribution and stratigraphic discontinuities. VII International Symposium on Mesozoic Terrestrial Ecosystem, Asociación Paleontológica Argentina, Publicación Especial 7, pp. 117-122. [ Links ]
6. Salgado, L., Coria, R.A., Magalhaes Ribeiro, C.M., Garrido, Alberto, Rogers, R., Simon, M.E., Arcucci, A.B., Curry-Rogers, K., Paulina Carabajal, A., Apesteguia, S., Fernandez, M. and Talevi, M. 2007. Upper Cretaceous dinosaur nesting site of Río Negro (Salitral Ojo de Agua and Salinas de Trapalcó-Salitral de Santa Rosa), northern Patagonia, Argentina. Cretaceous Research 28: 392-404. [ Links ]
7. Vianey-Liaud, M. and Crochet, J-Y. 1993. Dinosaur eggshells from the Late Cretaceous of Languedoc (Southern France). Revue de Paléobiologie, Vol. spec. 7, pp. 237-249. [ Links ]
Recibido: 19 de noviembre de 2008
Aceptado: 30 de septiembre de 2009.