versión ISSN 0327-9545
Biocell v.30 n.1 Mendoza ene./abr. 2006
Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allum cepa root model
R. Sehgal, S. Roy, and V.L. Kumar*
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi - 110 029, INDIA.
Address correspondence to: Dr. Vijay L. Kumar. Department of Pharmacology. All India Institute of Medical Sciences. Ansari Nagar, New Delhi-110 029, INDIA. Fax: (+91-11) 26588663. E-mail: firstname.lastname@example.org
Abstract: In the present study we have utilized the Allium cepa root tip meristem model to evaluate the cytotoxic and anti-mitotic activities of latex of Calotropis procera (DL) and podophyllotoxin. Standard cytotoxic drug cyclophosphamide and non-cytotoxic drugs cyproheptadine and aspirin served as controls. Like cyclophosphamide, both DL and podophyllotoxin significantly inhibited the growth of roots and mitotic activity in a dose-dependent manner. However, podophyllotoxin was more potent in this regard and produced root decay. Cyproheptadine and aspirin, on the other hand, showed a marginal effect on the root growth and mitotic activity at much higher concentrations.
Keywords: Calotropis procera; Podophyllotoxin; Cyclophosphamide; Cytotoxic; Allium cepa
A wide variety of anti-cancer drugs exhibit cytotoxic effect by interfering with cell-cycle kinetics. These drugs are effective against cells that are proliferating and produce cytotoxic effect either by damaging the DNA during the S-phase of the cell cycle or by blocking the formation of the mitotic spindle in M-phase (Gali-Muhtasib and Bakkar, 2002). An alkylating agent, cyclophosphamide, interferes with DNA integrity and thereby exhibits strong anti-mitotic activity both in vivo and in vitro (Crook et al., 1986; Misra and Bloom, 1991; Gereis et al., 1987). Most of the plant derived anti-cancer drugs affect the microtubule dynamics of the cell and induce persistent modification of biological processes and signaling pathways that ultimately lead to apoptotic death (Mollinedo and Gajate, 2003). Podophyllotoxin is one of such plant derived anti-mitotic agent that has been shown to produce mitotic arrest by changing the organization of mitotic spindle (Jordan et al., 1992). However, most of the cytotoxic drugs exhibit serious side effects (Powis, 1983). Hence, there is a need for drugs that are equally efficacious but have lesser side effects.
Calotropis procera, a wild growing plant is well known for its medicinal uses in traditional system of medicine for the treatment of variety of disease conditions that include leprosy, ulcers, tumors and piles (Kirtikar and Basu, 1935). The milky white latex obtained from the plant exhibits potent anti-inflammatory activity in various animal models that is comparable to standard anti-inflammatory drugs (Sangraula et al., 2002). It has been well established through various experimental and clinical studies that drugs possessing anti-inflammatory activity also exhibit anti-cancer properties (Umar et al., 2003). Earlier in vitro studies demonstrate potent cytotoxic activity in the flower extracts of Calotropis procera that is comparable to standard anticancer drug cisplatin (Smit et al., 1995). The present study was carried out to evaluate the cytotoxic and antimitotic potential of latex of C. procera and podophyllotoxin by standard assay method using Allium cepa root meristem model (Sharma, 1983). The effect was compared with standard anticancer drug cyclophosphamide and non-cytotoxic drugs cyproheptadine and aspirin.
Materials and Methods
Growing Allium cepa meristems
Locally available Allium cepa bulbs (50 ± 10 g) were grown in the dark over 100 ml tap water at ambient temperature until the roots have grown to approximately 2-3 cm. The water was changed daily.
Calotropis procera latex collection
The latex was collected from the aerial parts of C. procera growing in the wild. The plant was identified by the Raw Materials, Herbarium and Museum Division, National Institute of Science Communication, CSIR, New Delhi where a voucher specimen is preserved (Voucher No. PID 1739). The latex was dried under shade at ambient temperature (DL).
Conditions for drug Incubation
Working dilutions of all the drugs were made in tap water. DL was triturated in water to obtain 1 mg/ml and 10 mg/ml concentrations. Podophyllotoxin was initially dissolved in 100 ml ethanol and then diluted to required concentrations (0.05 mg/ml and 0.5 mg/ml) with water. Cyclophosphamide was used at 1 mg/ml and 10 mg/ml concentration while cyproheptadine and aspirin both were used at 5 mg/ml concentration. The bulbs with root tips grown upto 2-3 cms were placed over drug solutions and incubation was carried out at ambient temperature. The length of roots grown in drug solution (newly appearing roots not included), root number and the mitotic index were recorded at 0, 48 and 96 h and compared with that of control bulbs placed over tap water.
Microscopic examination and determination of mitotic index
The root tips (2-3 mms) were collected, placed in 1 N HCl for 5 minutes, squashed and finally stained with 2% aceto-orceine. For each root tip the number of mitotic and total meristematic cells were counted in 5-8 fields using high power (100X) light microscope (Badria et al., 2001). In all 400-500 cells were counted and cells manifesting different stages of mitosis i.e., interphase and prophase (P), metaphase (M), anaphase (A) and telophase (T) were recorded. The mitotic index was calculated using the following formula:
The values are given as mean ± SEM and the data was analyzed by Students t-test.
The inhibitory effect of podophyllotoxin and DL was evaluated on the growth and mitotic activity of Allium cepa root meristems and the effect was compared with standard anti-cancer drug cyclophosphamide. A progressive increase in root number and root length was observed in control group. The root length in control group at 0, 48 and 96 h was 2.80±0.28 cm (n=12), 3.09±0.22 cm (n=24) and 3.82±0.19 cm (n=34). Incubation of bulbs in different concentrations of cytotoxic agents produced a growth retarding effect that was associated with a decrease in the root number (Fig. 1). Both DL and cyclophosphamide arrested the root growth. However, the root number did not increase any further at 10 mg/ml concentration. Podophyllotoxin, on the other hand, produced root decay and decreased the root length and root number significantly at 48 h and 96 h as compared to that at 0 h (p<0.001). The root length at 0.5 mg/ml of podophyllotoxin was 2.72±0.18 cm (n=13), 1.46±0.22 cm (n=8) and 1.00±0.05 cm (n=3) at 0 h, 48 h and 96 h, respectively. Non-cytotoxic drugs, cyproheptadine and aspirin, did not produce a change in the root growth and root number (Table 1).
FIGURE 1. Allium cepa bulbs showing the effect of various cytotoxic agents on root length following 96 h of incubation. A: control; B: DL (10 mg/ml); C:Podophyllotoxin (0.5 mg/ml); D: cyclophosphamide (10 mg/ml)
The mitotic cells were counted in the root meristems in above groups at 0, 48 and 96 h of incubation with each drug. The mitotic index ranged between 60.7±0.7 and 63.0±2.3 in the control group over a period of 96 h. DL produced a significant decrease in mitotic index that was dose and time dependent. The mitotic index at 10 mg/ml concentration of DL was 32.7±0.8 at 48 h as compared to 57.6±0.4 at 0 h while at 96 h the cellular morphology was lost. Treatment with 0.05 mg/ml of podophyllotoxin significantly reduced the mitotic index to 43.9±2.5 at 96 h (p<0.01) while at higher concentration the cellular morphology was lost and the roots decayed. Treatment with cyclophosphamide at 1 mg/ml and 10 mg/ml concentration brought down the mitotic index to 40.6±1.3 and 37.3±1.0 at 96 h (p<0.0001). Cyproheptadine and aspirin produced only a marginal decrease in mitotic index at 5 mg/ml concentration (Table 2).
Allium cepa root tip meristems have been widely used for the evaluation of cytotoxic and anti-mitotic activity of various compounds (Shehab, 1980; Williams and Omoh, 1996; Al-Meshal, 1987). In the present study we have tested the cytotoxic and anti-mitotic effect of DL, standard anticancer drugs, podophyllotoxin and cyclophosphamide in Allium cepa root tip meristem model and compared it with two non-cytotoxic drugs cyproheptadine and aspirin. Podophyllotoxin, an inhibitor of microtubule assembly was most effective in inhibiting mitosis in Allium cepa root tip meristems. In this model, its cytotoxic effect was evident in the form of shortening and decaying of roots. Anti-tumor drugs that interact with microtubules and tubulin are known to block mitosis and induce cell death by apoptosis (Jordan, 2002). The cytotoxic effect of DL was comparable to that of cyclophosphamide and both agents inhibited root growth and mitosis to a significant extent. Earlier the extracts prepared from the dried flowers, roots and leaves of C. procera have been reported to exhibit potent cytotoxic activity (Smit et al., 1995; Hussein Ayoub and Kingstonb, 1981). This activity has been attributed to the cardinolide calotropin that is present in the latex as well (Kupchan et al., 1964; Kiuchi et al., 1998). Further, the DL might be inhibiting growth factor mediated mitogenic signaling pathways. On the other hand, cyclophosphamide alkylates DNA and proteins after it has been metabolized by cytochrome P450 to yield phosphoramide mustard and acrolein. It induces plasma membrane blebbing, DNA fragmentation and cleavage of poly (ADP-ribose) polymerase (PARP) and produces cell death by apoptosis (Schwartz and Waxman, 2001).
It is important to note that non-cytotoxic drugs, cyproheptadine and aspirin did not affect the root length and root number. Both drugs also produced marginal antimitotic effect at a dose that was much higher than that of anti-cancer drugs. At higher concentration cyproheptadine has been shown to suppress cell division and exhibit cytotoxic effect of non-specific nature (Zang et al., 1975). The cytotoxic effects of aspirin, an anti-inflammatory drug, are mainly due to its ability to induce DNA fragmentation and proteolytic cleavage of PARP. It is also known to activate caspases through cyclooxygenase-independent mechanism (Bellosillo et al., 1998).
Thus, our study demonstrates that latex of C. procera exhibits cytotoxic properties like standard anticancer drugs podophyllotoxin and cyclophosphamide. However, the mechanism for such an effect needs further evaluation.
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Received on December 21, 2004.
Accepted on December 13, 2005.