SciELO - Scientific Electronic Library Online

 
vol.77 issue3Effect of bagging and “deschire” of the banana (Musa acuminata Colla) crop on the populations of thrips (Thysanoptera: Thripidae) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

Share


Revista de la Sociedad Entomológica Argentina

Print version ISSN 0373-5680On-line version ISSN 1851-7471

Rev. Soc. Entomol. Argent. vol.77 no.3 La Plata Sept. 2018

 

Artículo-Article

Diversity of Ceratopogonidae (Diptera: Culicomorpha) from the Reserva Natural Integral Punta Lara (Buenos Aires, Argentina)

Diversidad de Ceratopogonidae (Diptera: Culicomorpha) de la Reserva Natural Integral Punta Lara (Buenos Aires, Argentina)

 

CAZORLA, Carla G.1*, MARINO, Pablo I.1, DÍAZ, Florentina2 & CAMPOS, Raúl E.3

1 División Entomología, Museo de La Plata, Paseo del Bosque s/n (B1900FWA) La Plata, Buenos Aires, Argentina, and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). * E-mail: carlacazorla@fcnym.unlp.edu.ar
2 Centro de Estudios Parasitológicos y de Vectores (CEPAVE), CONICET-UNLP. La Plata, Buenos Aires, Argentina.
3 Instituto de Limnología “Dr. Raúl A. Ringuelet”. CONICET-UNLP. La Plata, Buenos Aires, Argentina. Received 08 - V - 2018 | Accepted 21 - VIII - 2018 | Published 27 - IX - 2018 https://doi.org/10.25085/rsea.770301

 


ABSTRACT. We present the results of the frst survey on the Ceratopogonidae from the “Reserva Natural Integral Punta Lara”, in Buenos Aires province. This protected area represents the southernmost relict of gallery forest where the knowledge of the insect fauna is still limited. Catches were conducted in areas of grassland and marginal forest with Malaise traps, CDC light traps and funnel light traps. A total of 4,195 specimens belonging to nine genera and 39 species of Ceratopogonidae were identifed. A list of the species present in the protected area is provided, along with their distributional data. Twenty-three species are recorded from the reserve for the frst time, while Dasyhelea mediomunda Minaya, Forcipomyia ( Forcipomyia) taragui Marino, Spinelli & Cazorla, Forcipomyia ( Microhelea) fuliginosa (Meigen), Stilobezzia (Eukraiohelea) elegantula (Johannsen) and Stilobezzia (Stilobezzia) punctulata Lane are recorded for the frst time in Buenos Aires province.

KEYWORDS. Biting midges. First record. Gallery forest. Grassland. Protected area.

RESUMEN. Se presenta el primer relevamiento de Ceratopogonidae de la Reserva Natural Integral Punta Lara, en la provincia de Buenos Aires. Este área protegida representa uno de los relictos más australes de la selva en galería, donde el conocimiento de la fauna de insectos es aún limitado. Las capturas se realizaron en ambientes de pastizal y selva en galería, con trampas Malaise y trampas de luz, tipo CDC y embudo. Un total de 4.195 especímenes pertenecientes a nueve géneros y 39 especies de Ceratopogonidae fueron identifcados. Se brinda un listado de las especies presentes en el área, y se proveen datos de su distribución geográfca. V eintitrés especies se citan en la Reserva por primera vez, mientras que Dasyhelea mediomunda Minaya, Forcipomyia (Forcipomyia) taragui Marino, Spinelli & Cazorla, Forcipomyia (M icrohelea) fuliginosa (Meigen), Stilobezzia (Eukraiohelea) elegantula (Johannsen) y Stilobezzia (Stilobezzia) punctulata Lane se registran por primera vez en la provincia de Buenos Aires.

PALABRAS CLAVE. Área protegida. Jejenes. Pastizal. Primer registro. Selva en galería.


 

INTRODUCTION

The Ceratopogonidae or biting and predaceous midges, commonly known in Argentina as "jejenes", are a large and diverse family of small fies. They are common inhabitants of fresh and saline lentic and lotic ecosystems on all continents except Antarctica (Borkent & Wirth, 1997). There are more than 1,100 known species in the Neotropical region, while in Argentina at least 255 species belonging to 32 genera have been recorded, 47 of which were found in the province of Buenos Aires (Borkent & Spinelli, 2007; Spinelli et al., 2010; Marino et al., 2011; Ronderos et al., 2011, 2017; Borkent, 2016; Cazorla, 2016).

From a biogeographical point of view, Argentina spans over both the Andean and Neotropical regions, including part of the South American transition zone (Morrone, 2014). In the Neotropical region, the Chacoan sub–region includes among others the Chacoan dominion, which is divided in Argentina into two provinces: Chaco and Pampean provinces. The Reserva Natural Integral Punta Lara (RNIPL) is located in the Pampean province. Moreover, from an ecological point of view the RNIPL occupies two ecoregions: Delta e Islas del Paraná and Pampa (Dinerstein et al., 1995). The RNIPL was declared main area of the Biosphere Reserve " Pereyra Iraola" ( UNESCO, 2017) and it is a protected natural area within a system of protected areas of the province. It was created in 1949 and its main roles are the conservation of the biodiversity of the southernmost relict of the gallery forest, the environmental education and interpretation, and the scientifc research.

Despite being the most diverse animal group, arthropods have been and remain minimally represented in biodiversity inventories; insects, as the most species–rich and ecologically varied and complex components of animal biodiversity, cannot be disregarded in assessing impacts of the environmental changes ( New, 2012). Knowledge about the insect fauna of the RNIPL is limited. Most systematic and ecological studies date back to the year 2000, such as the ones concerning the orders Coleoptera ( e.g. : Fernández, 1995; Von Ellenrieder & Fernández, 2000), Odonata ( e.g. : Rodrigues Capítulo, 1988, 1996; Rodrigues Capítulo & Muzón, 1990; Von Ellenrieder, 2000), and Heteroptera ( e.g.: Schnack et al., 1986, 1989; Von Ellenrieder & Perez Goodwyn, 2000). As regards to the Diptera, only three families have been briefy studied in this area: Ceratopogonidae ( Spinelli et al., 1989), Chironomidae ( Donato & Paggi, 2005; Siri et al., 2008; Mauad et al., 2013) and Culicidae ( e.g. : García et al., 1994, 1995; Maciá et al., 1995, 1997; Campos & Zavortink, 2010). In order to manage and evaluate the effectiveness of protected areas to preserve biodiversity, it is important to have a comprehensive knowledge of the species under protection ( Vieira et al., 2013). This study presents an updated list of species of Ceratopogonidae from the Reserva Natural Integral Punta Lara, in the province of Buenos Aires, Argentina.

MATERIAL AND METHODS

Study site

The Reserva Natural Integral Punta Lara (34º 47’ 18” S; 58º 00’ 01” W) has an extension of 6,000 ha in northeast of Buenos Aires province (Fig. 1), on the coast of the estuary of the Río de La Plata within the localities of Ensenada and Berazategui. The plant communities that make up the Reserve are forest, wetlands and grasslands. The forest extends along streams and river banks, which earned it the name of marginal forest. The wetlands are composed of riverside scrubland, lagoons, streams, clipper grassland, lilygrass and channels. The grassland occupies much of the Reserve with a marked heterogeneity of environments determined by geomorphological and edaphic differences (Herrera & Torres Robles, 2012).

Data collection

Catches of adult Ceratopogonidae were conducted between July 2014 and February 2017 with CDC light traps, funnel light traps, and Malaise traps. Traps were installed in different points within the marginal forest and in the neighboring open grassland. CDC light traps were installed in the marginal forest and worked for ffteen continuous hours from sunset, once a month during one year ( September 2014 to October 2015); Malaise and funnel traps were installed in the marginal forest as well as in the grassland, and worked during two continuous weeks in each point, every month along one year (February 2016 to March 2017), although collector cup removal was carried out every seven days. In the laboratory, specimens were separated by genus with the aid of a stereo microscope. To identify them, at least one male and one female of each species were mounted on slides with Canada balsam according to Borkent & Spinelli ( 2007), and identifed based on diagnostic keys and original descriptions. The remaining specimens were preserved in ethyl alcohol 70%. For the recorded species the citation of the original description is presented, followed by the previous records from Buenos Aires province. The known distribution of the species refers to the political division of Argentina into provinces where the species have been recorded in the literature ( Table I), plus the detailed localities in the case of Buenos Aires province. The order of taxa follows the Neotropical catalog of Ceratopogonidae ( Borkent & Spinelli, 2007). Studied specimens are deposited in the Entomological collection of the Museo de La Plata, Buenos Aires, Argentina (MLP).

RESULTS

We list all taxa determined to species level, belonging to subfamilies Forcipomyünae, Dasyheleinae and Ceratopogoninae.

Family Ceratopogonidae

Subfamily Forcipomyiinae Lenz, 1934

Genus Atrichopogon Kieffer

Atrichopogon (Atrichopogon) albinensis Ingram & Macfie, 1931

Atrichopogon albinensis Ingram & Macfe, 1931: 228 (male, female; Argentina); Ronderos et al., 2011: 1186.

Known distribution: Argentina: Buenos Aires: Bahía Blanca, Isla Martín García and RNIPL (new record).

Material examined: 2♀, marginal forest, 10-11/XII/ 2014, CDC light trap; 1♀, marginal forest, 22/II-1/III/ 2017, funnel light trap; 3♀ 1♂, same data except 7-14/ VIII/2016, Malaise trap; 2♀, grassland, 22-29/VI/2016, Malaise trap; 1♀, same data except 27/VII-3/VIII/2016; 1♂, same data except 17-24/X/2016; 4♀, same data except 24-31/VIII/2016; 1♀, same data except 2-9/III/ 2016, funnel light trap. Cazorla, C. G. & Campos, R. E. cols.

Atrichopogon (Atrichopogon) balseiroi Spinelli, 1982

Atrichopogon balseiroi Spinelli, 1982: 206 (male, female; Argentina).

Known distribution: Argentina: Buenos Aires: Berisso and RNIPL (new record).

Material examined: 1♂, marginal forest, 3-4/II/2015, CDC light trap; 2♀ 1♂, marginal forest, 15/V-6/VI-2015, funnel light trap, 11♀ 1♂, same data except 22/II-1/III/ 2017. Cazorla, C. G. & Campos, R. E. cols.

Atrichopogon (Atrichopogon) comechingon Spinelli & Marino, 2006

Atrichopogon (Atrichopogon) comechingon Spinelli & Marino, in Spinelli et al., 2006: 306 (male, female; Argentina); Marino et al., 2011: 200.

Known distribution: Argentina: Buenos Aires: RNIPL (new record) and Sierra de la Ventana; Córdoba, La Pampa, La Rioja, San Luis and Río Negro.

Material examined: 2♀, marginal forest, 9-11/IX/2014, CDC light trap; 1♀, same data except 6-7/IX/2014; 1♂, same data except 12-13/III/2015; 1♀, marginal forest, 12-18/II/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.


Fig. 1. Geographical location of Reserva Natural Integral Punta Lara and sampling sites in Buenos Aires province, Argentina.

 

Atrichopogon (Lophomyidium) delpontei Cavalieri

& Chiossone, 1972

Atrichopogon (Atrichopogon) homofacies Atrichopogon delpontei Cavalieri & Chiossone, 1972:

Spinelli, 1989                                                                       121 (male, female; Argentina); Spinelli & Balseiro, 1982:

Atrichopogon homofacies Spinelli, in Spinelli et al., 226.

1989: 734 (male, female; Argentina); Ronderos et al.,

Atrichopogon (Lophomyidium) delpontei: Marino et

2011: 1186. al., 2017: 2082.

Known distribution: Argentina: Buenos Aires: Isla        Known distribution: Argentina: Buenos Aires: Berisso

Martín García and RNIPL.                                                     and RNIPL (new record), Formosa, Chaco and Santa Fe.

Material examined: 2♀ 2♂, marginal forest, 9-10/IX/    Brazil

2014, CDC light trap; 2♀ 1♂, same data except 3-10/       Material examined: 1♀ 1♂, marginal forest 9-10/IX/

VIII/2016, Malaise trap; 1♀ 2♂, same data except 10-17/   2014, CDC light trap; 1♀ 1♂, same data except 10-11/

VIII/2016; 10♀ 9♂, grassland, 12-19/X/2016, Malaise   XII/2014; 1♀, marginal forest, 18-25/I/2017, funnel light

trap; 2♂, same data except 8-15/II/2017. Cazorla, C. G.    trap; 1♀, same data except 25/I-1/II/2017; 1♀,

& Campos, R. E. cols.                                                           grassland, 14-21/XII/2016, funnel light trap; 2♀ 3♂,

marginal forest, 30/XI-7/XII/2016, funnel light trap.

Atrichopogon (Atrichopogon) seudoobfuscatus

Cazorla, C. G. & Campos, R. E. cols.

Spinelli, 1982

Atrichopogon seudoobfuscatus Spinelli, 1982: 208 Atrichopogon (Psilokempia) domizii Spinelli, 1982

(male, female; Argentina).

Atrichopogon domizii Spinelli, 1982: 206 (male,

Known distribution: Argentina: Buenos Aires: Berisso

female; Argentina).

and RNIPL (new record).

Atrichopogon (Psilokempia) domizii: Spinelli et al.,

Material examined: 2♂, marginal forest, 9-10/X/2014,    2015: 18. CDC light trap; 1♀, same data except 6-7/X/2014; 1♀ Known distribution: Argentina: Buenos Aires: Berisso,

1♂, same data except 10-11/XII/2014; 1♂, grassland,    Campana and RNIPL (new record). 7-14 XII/2016, Malaise trap; 1♂, marginal forest, 23-30/ Material examined: 1♂, marginal forest, 10-11/XII/

XI/2016, funnel light trap. Cazorla, C. G. & Campos, R.    2014, CDC light trap. Cazorla, C. G. & Campos, R. E.

Atrichopogon (Atrichopogon) talarum Spinelli, 1982

Atrichopogon talarum Spinelli, 1982: 204 (male, female; Argentina).

Known distribution: Argentina: Buenos Aires: Berisso and RNIPL.

Material examined: 3♀ 1♂, marginal forest, 10-11/ XI/2014, CDC light trap; 1♂, same data except 9-10/ XII/2014; 1♂, same data except 12-13/III/2015; 1♀ 1♂, same data except, 23-30/XI/2016, funnel light trap; 2♀ 3♂, same data except 30/X-7/XI/2016; 8♀ 9♂, same data except 22/II-1/III/2017, Malaise trap; 1♀, grassland, 7-14/XII/2016, Malaise trap; 1♀ 1♂, same data except 11-18/I/2017, funnel light trap. Cazorla, C. G. & Campos, R. E. cols.

Atrichopogon(Atrichopogon) sp. nov. A

Material examined: 2♀, marginal forest 6-7/X/2014, CDC light trap; 38♀ 20♂, same data except 10-11/XI/ 2014; 8♀ 2♂, same data except 7-8/I/2015; 3♂, same data except 3-4/2015, 1♀, grassland, 4-12/V/2016, Malaise trap; 2♀, same data except 12-19/X/2016; 1♀ 1♂, marginal forest, 23-30/XI/2016, funnel light trap. Cazorla, C. G. & Campos, R. E. cols.

Genus Forcipomyia Meigen

Forcipomyia (Euprojoannisia) platensis (Brèthes, 1914)

Euprojoannisia platensis Brèthes, 1914: 156 (female; Argentina).

Forcipomyia (Euprojoannisia) platensis Brèthes: Wirth & Cavalieri, 1975: 125 (combination); Spinelli, 1983a: 127.

Known distribution: Argentina: Buenos Aires: Berisso, Escobar, General Urquiza, RNIPL (new record) and Tigre.

Material examined: 1♀, marginal forest, 12-13/III/ 2015, CDC light trap; 1♀, same data except 7-8/V/2015; 4♀, grassland, 4-21/XII/2016, Malaise trap; 2♀, same data except 16-23/XI/2016. Cazorla, C. G. & Campos, R. E. cols.

Forcipomyia (Forcipomyia) pictoni Macfie, 1938

Forcipomyia pictoni Macfe, 1938: 161 (male; Trinidad).

Forcipomyia (Forcipomyia) pictoni: Wirth, 1974: 6; Ronderos et al., 2011: 1186.

Known distribution: Argentina: Buenos Aires: Isla Martín García and RNIPL (new record), Corrientes, Formosa, La Rioja and Misiones. Belize, Brazil, Honduras, Costa Rica, Jamaica, Mexico, Panama, Trinidad, United States of America and Venezuela.

Material examined: 1♀, marginal forest, 22/II-1/III/ 2017, funnel light trap. Cazorla, C. G. & Campos, R. E. cols.

Forcipomyia(Forcipomyia) pinamarensisSpinelli, 1983

Forcipomyia (Forcipomyia) pinamarensis Spinelli, 1983a: 121 (male, female; Argentina).

Known distribution: Argentina: Buenos Aires: Campana, Pinamar and RNIPL (new record). Brazil, Costa Rica, Panama, Uruguay and Venezuela.

Material examined: 16♀ 4♂, marginal forest 9-10/IX/ 2014, CDC light trap; 2♀, same data except 6-7/X/2014; 4♂, same data except 10-11/XII/2014; 1♀ 1♂, same data except 13-20/IV/2016, Malaise trap; 7♀, same data except funnel light trap; 33♀ 8♂, grassland, 9-16/2016, Malaise trap; 10♀ 8♂, same data except 27/IV-4/V/ 2016; 9♀ 34♂, marginal forest, 22/II-1/III/2017, funnel light trap; 1♂, same data except Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

Forcipomyia (Forcipomyia) poulaineae Ingram & Macfie, 1931

Forcipomyia poulaineaeIngram & Macfe, 1931: 227 (male; Argentina).

Forcipomyia (Forcipomyia) poulaineae: Spinelli & Balseiro, 1982: 226; Spinelli 1983a: 124; Ronderos et al., 2011: 1187; Marino et al., 2011: 202.

Known distribution: Argentina: Buenos Aires: Buenos Aires city, Berisso, Isla Martín García, RNIPL and Sierra de la Ventana; Entre Ríos. Brazil.

Material examined: 2♀, grassland, 27/III-4/V/2016, Malaise trap; 3♀ 3♂, same data except 24-31/VIII/2016; 1♂, marginal forest, 13-20/VII/2016, funnel light trap; 1♀, same data except 3-10/VIII/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

Forcipomyia (Forcipomyia) rioplatensis Marino & Spinelli, 2002

Forcipomyia squamitibia Lutz: Spinelli, 1983a: 128 (female, male; Argentina).

Forcipomyia (Forcipomyia) rioplatensisMarino & Spinelli, 2002: 314 (male, female; Argentina); Spinelli et al., 2005: 109; Ronderos et al., 2011: 1187; Marino et al., 2011: 202.

Known distribution: Argentina: Buenos Aires: Berisso, Isla Martín García, RNIPL, San Vicente, Sierra de la Ventana, and Córdoba. Uruguay.

Material examined: 4♀ 1♂, grassland, 12-19/X/2016, Malaise trap; 1♀ 3♂, same data except 1-8/II/2017. Cazorla, C. G. & Campos, R. E. cols.

Forcipomyia(Forcipomyia)taraguiMarino, Spinelli & Cazorla, 2002

Forcipomyia(Forcipomyia) taraguiMarino et al, 2002: 17 (new name for guarani).

Forcipomyia(Forcipomyia) guaraniMarino & Spinelli,

1999a: 448 (male, female; Argentina).

Known distribution: Argentina: Buenos Aires: RNIPL (new record), Corrientes and Misiones. Paraguay and Uruguay.

First record in Buenos Aires province.

Material examined: 1♀, marginal forest, 10-11/XI/ 2014, CDC light trap; 1♀, same data except 3-4/II/ 2015; 5♀ 1♂, same data except 12-13/III/ 2015; 1♀, marginal forest, 13-20/2016, Malaise trap; 1♀ 3♂, marginal forest, 22-II-1/III/2017, funnel light trap. Cazorla, C. G. & Campos, R. E. cols.

Forcipomyia (Lepidohelea) edmistoni Wirth & Spinelli, 1993

Forcipomyia (Lepidohelea) edmistoni Wirth & Spinelli, 1993: 624 (larva, pupa, male, female; USA); Spinelli & Marino, 1998: 39 (Argentina record).

Known distribution: Argentina: Buenos Aires: RNIPL. United States of America.

Material examined: 1♂, marginal forest, 9-1/IX/2014, CDC light trap; 1♀, same data except 10-11/XI/2014; 1♀, same data except 10-11/XII/2014; 1♀, same data except 7-14/2016, Malaise trap; 2♀ 1♂, same data except 30/XI-7/XII/2016. Cazorla, C. G. & Campos, R. E. cols.

Forcipomyia (Metaforcipomyia) williamsi Marino & Spinelli, 1999

Forcipomyia (Metaforcipomyia) williamsi Marino & Spinelli, 1999b: 5 (female, male; Argentina).

Known distribution: Argentina: Buenos Aires: Berisso, Isla Martín García and RNIPL.

Material examined: 5♀, marginal forest, 6-7/X/2014, CDC light trap. Cazorla, C. G. & Campos, R. E. cols.

Forcipomyia (Microhelea) fuliginosa (Meigen, 1818)

Ceratopogon fuliginosus Meigen, 1818: 86 (female; Germany).

Forcipomyia fuliginosa Goetghebuer, 1933: 130 (combination).

Forcipomyia (Microhelea) fuliginosa (Meigen): Wirth, 1972: 567.

Forcipomyia crudelisKnab, 1914: 66.

Ceratopogon tropica(Kieffer), 1917: 297.

Known distribution: Argentina: Buenos Aires: RNIPL (new record) and Misiones. Brazil, Colombia, Costa Rica, Trinidad, Uruguay and United States of America. Widespread in Palearctic, Oriental and Afrotropical regions.

First record in Buenos Aires province.

Material examined: 2♀, marginal forest, 10-11/XI/ 2014, CDC light trap; 3♀, same data except 10-11/XII/ 2014; 1♀, same data except 7-8/I/2015; 1♀, same data except 3-4/II/2015; 1♂, same data except 12-13/III/2015. Cazorla, C. G. & Campos, R. E. cols.

Forcipomyia (Saliohelea) leei Wirth & Ratanaworabhan, 1978

Forcipomyia (Saliohelea) leei Wirth & Ratanaworabhan, 1978: 498 (male, female, pupa; Colombia); Marino et al., 2011: 202.

Known distribution: Argentina: Buenos Aires: RNIPL (new record) and Sierra de la Ventana. Brazil, Colombia, Costa Rica, Puerto Rico and United States of America.

Material examined: 1♀, marginal forest, 8- 9/VI/2015, CDC light trap. Cazorla, C.G. & Campos, R.E. cols.

Forcipomyia(Thyridomyia) nana(Macfie, 1939)

Lasiohelea nanaMacfe, 1939: 171 (female; Brazil).

Forcipomyia (Thyridomyia) nana (Macfe): Wirth, 1956: 247 (combination); Spinelli et al., 2010: 130.

Known distribution: Argentina: Buenos Aires: RNIPL. Brazil, Colombia, Costa Rica, Dominica, Ecuador, Guatemala, Jamaica, Mexico, Panama, Trinidad and Virgin Islands.

Material examined: 1♂, marginal forest, 3-4/II/2015, CDC light trap. Cazorla, C. G. & Campos, R. E. cols.

Subfamily Dasyheleinae Lenz, 1934

Genus Dasyhelea Kieffer

Dasyhelea mediomunda Minaya, 1978

Dasyhelea mediomunda Minaya, 1978: 79 (male, female, pupa; Peru).

Known distribution: Argentina: Buenos Aires: RNIPL (new record), Chubut, Neuquén and Rio Negro. Chile.

First record in Buenos Aires province

Material examined: 1♂, grassland, 9-16/III/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

Dasyhelea necrophila Spinelli & Rodríguez, 1999

Dasyhelea necrophila Spinelli & Rodríguez, 1999: 59 (male, female, pupa, larva; Argentina); Ronderos et al., 2011: 1187; Marino et al., 2011: 203.

Known distribution: Argentina: Buenos Aires: Bahía Blanca, Berisso, La Plata (Gonnet), Isla Martín García, RNIPL (new record) and Sierra de la Ventana.

Material examined: 1♂, grassland, 9-16/III/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

Dasyhelea serrana Díaz & Spinelli, 2014

Dasyhelea serrana Díaz & Spinelli, in Díaz et al., 2014: 2153 (male, female, pupa; Argentina).

Known distribution: Argentina: Buenos Aires: RNIPL (new record) and Sierra de la Ventana; Río Negro.

Material examined: 1♂, marginal forest, 10-11/XIII/ 2014, CDC light trap. 1♂, grassland, 27/III-4/IV/2016, Malaise trap; 5♂, same data except 7-14/XIII/2016; 1♀1♂, same data except 1-8/III/2017, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

Dasyhelea suareziSpinelli & Ronderos, 1987

Dasyhelea suarezi Spinelli & Ronderos, 1987: 11 (male, female; Argentina).

Known distribution: Argentina: Buenos Aires: Berisso and RNIPL (new record).

Material examined: 1♂, marginal forest, 7-8/I/ 2015, CDC light trap. Cazorla, C. G. & Campos, R. E. cols.

Subfamily Ceratopogoninae Newman, 1834

Tribe Culicoidini Kieffer, 1911

Genus Culicoides Latreille

Culicoides (Cotocripus) caridei (Brèthes, 1912)

C otocripus caridei Brèthes, 1912: 451 (female; Argentina).

Culicoides caridei (Brèthes): Lane, 1945: 366; Spinelli et al., 1989: 735; Ronderos & Spinelli, 1990: 83; Ronderos et al., 2011: 1187.

Centrorrhynchus setiferLutz, 1913: 64.

Dasyhelea carideiLane & Ortíz, 1950: 460.

Known distribution: Argentina: Buenos Aires: Berisso, Coronel Pringles, La Plata (Gonnet), Isla Martín García, Magdalena and RNIPL; Jujuy. Uruguay.

Material examined: 60♀ 72♂, marginal forest, 30/XI-7/ XIII/2016, funnel light trap; 67♀, 4♂, grassland, 7-14/XII/ 2016, funnel light trap; 5♀, same data except 14-21/XIII/ 2016; 1♀, same data except 26/V- 6/VI/2016. Cazorla, C. G. & Campos, R. E. cols.

Culicoides (Haematomydium) debilipalpis Lutz, 1913

Culicoides debilipalpisLutz 1913: 60 (female, Brazil); Ronderos et al., 2011: 1188.

Culicoides ichesi Ronderos & Spinelli, 1995: 77.

Culicoides khalafBeck, 1957: 104.

Culicoides lahilleiSpinelli & Wirth, 1986: 62.

Known distribution: Argentina: Buenos Aires: Berisso, Isla Martín García and RNIPL; Corrientes, Entre Ríos, Jujuy, Misiones, Salta and Tucuman. Belize, Brazil, Colombia, Costa Rica, Honduras, Guatemala, Panama, Peru, Trinidad, Uruguay, Venezuela and United States of America.

Material examined: 6♀ 1♂, marginal forest, 30/XI-7/ XII/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

Culicoides(Hoffmania) insignisLutz, 1913

Culicoides insignis Lutz 1913: 51 (male, female, pupa; Brazil); Spinelli & Balseiro, 1982: 226; Spinelli et al., 1989: 735; Ronderos et al., 2011: 1188.

Culicoides inamollaeFox & Hoffman, 1944: 110.

Culicoides painteriFox, 1946: 257.

Known distribution: Argentina: Buenos Aires: Berisso, Isla Martín García and RNIPL; Chaco, Corrientes, Entre Ríos, Formosa, Misiones, Jujuy, Salta and Tucumán.

Brasil, Cayman Islands, Colombia, Costa Rica, Cuba, Dominica, Dominican Republic, Ecuador, French Guiana, Guadeloupe, Haiti, Honduras, Jamaica, Mexico, Panama, Paraguay, Puerto Rico, St. Lucía, Trinidad, Venezuela, United States of America and Uruguay.

Material examined: 1♀, marginal forest, 10-11/XI/ 2014, CDC light trap; 6♀ 1♂, same data except 10-11/ XII/2014; 1♀, same data except 3-4/II/2015; 1♀, same data except 12-13/III/2015; 1♀, same data except 6-7/ V/2015; 1♂, marginal forest, 12-18/II/2016, funnel light trap; 1♀, grassland, 26/V-6/VI/2016, Malaise trap; 1♂, same data except, 14- 21/XII/2016, funnel light trap. Cazorla, C. G. & Campos, R. E. cols.

Culicoides (Psychophaena) venezuelensis Ortíz & Mirsa, 1950

Psychophaena pictipennis (Philippi), 1865: 628 (female; Chile).

Culicoides venezuelensis Ortíz & Mirsa, 1950: 137 (male, female; Venezuela); Wirth, 1955: 234 (= pictipennis Philippi 1865, non Steager, 1839; = ortizi Fox); Spinelli & Balseiro 1982: 226; Spinelli & Wirth, 1986: 64; Spinelli et al., 1989: 735; Ronderos et al., 2011: 1189; Marino et al., 2011: 203.

Culicoides ortiziFox, 1952: 366.

Known distribution: Argentina: Buenos Aires: Berisso, Isla Martín García, RNIPL and Sierra de la Ventana; Catamarca, Chaco, Córdoba, Corrientes, Entre Ríos, Formosa, Jujuy, Mendoza, Misiones, Río Negro, Salta, Santiago del Estero and Tucumán. Brazil, Bolivia, Chile, Colombia, Costa Rica, Ecuador, Panamá, Paraguay, Uruguay and Venezuela.

Material examined: 1♀, marginal forest, 10-11/XI/ 2014, CDC light trap; 15♀ 13♂, same data except 10-11/ XII/2014; 2♂, same data except 7-8/I/ 2015; 23♀ 2♂, same data except 3-4/II/2015; 1♀ 2♂, same data except 12-13/III/2015; 2♀ 1♂, same data except 6-7/V/2015; 5♀ 7♂, same data except, 22/II-1/III/2017, funnel light trap; 27 ♀ 1♂, same data except 23-30/XI/2016; 18♀ 1♂, same data except 30/XI-7/XII/2016; 2♀; same data except 28/XII- 4/VI/2017; 5♀ 7♂, same data except 22/ II-1/III/2017; 1♀, grassland, 9-16/III/2016, Malaise trap; 1♀, same data except 14-21/XII/2016, funnel light trap; 1♂, same data except 8-15/II/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

Tribe Ceratopogonini

Genus Alluaudomyia Kieffer

Alluaudomyia schnacki Spinelli, 1983

Alluaudomyia schnacki Spinelli, 1983b: 403 (larva, pupa, male, female; Argentina); Spinelli et al., 1989; Marino et al., 2011: 203; Díaz et al., 2015: 89.

Known distribution: Argentina: Buenos Aires: Sierra de la Ventana, Berisso, Escobar and RNIPL; Chubut, Corrientes, Entre Ríos, Formosa. Chile and Uruguay.

Material examined: 5♀ 1♂, marginal forest, 10-11/XII/ 2014, CDC light trap; 3♀, same data except 22/II-1/III/ 2017, funnel light trap; 5♀, 3♂, 29/I-5/II/2016, grassland, Malaise trap; 1♂, same data except 19-26/XII/2016, funnel light trap; 1♀, same data except Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

Genus Stilobezzia Kieffer

Stilobezzia (Eukraiohelea) elegantula (Johannsen, 1907)

Bezzia elegantula Johannsen, 1907: 109 (female; United States of America).

Probezzia elegantula: Malloch, 1914a: 137.

Parabezzia elegantula Malloch, 1915: 359.

Parabezzia (Eukraiohelea) elegantula Johannsen, 1934: 345.

Eukraiohelea elegantula Johannsen, 1943: 781.

Stilobezzia (Eukraiohelea) elegantula Wirth, 1953: 62.

Stilobezzia (Eukraiohelea) maculitibia Lane & Forattini, 1956: 207.

Stilobezzia subsessilisKieffer, 1917: 311.

Known distribution: Argentina: Buenos Aires: RNIPL (new record), Chaco and Misiones. Brazil, Mexico, Panama, Paraguay, (?) Puerto Rico and United States of America.

First record in Buenos Aires province.

Material examined: 1♀, marginal forest, 10-11/XII/

2014, CDC light trap. Cazorla, C. G. & Campos, R. E. cols.

Stilobezzia (Stilobezzia) bicolor Lane, 1947

Stilobezzia (Stilobezzia) bicolor Lane, 1947: 208 (male, Brazil); Spinelli, 1983b: 410.

Known distribution: Argentina: Buenos Aires: Berisso and RNIPL (new record). Brazil and Panama.

Material examined: 1♀, marginal forest, 12-13/III/

2015,  CDC light trap. Cazorla, C. G. & Campos, R. E cols.

Stilobezzia (Stilobezzia) fiebrigi Kieffer, 1917

Stilobezzia febrigi Kieffer, 1917: 309 (female, Paraguay).

Stilobezzia (Stilobezzia) febrigi Forattini & Rabello, 1956: 45; Spinelli & Balseiro, 1982: 226; Spinelli, 1983b: 410.

Known distribution: Argentina: Buenos Aires: Berisso, RNIPL and Tigre, Corrientes, Entre Ríos and Santiago del Estero. Brazil and Paraguay.

Material examined: 1♀ 1♂, marginal forest, 10-11/XI/ 2014, CDC light trap; 1♀ 6♂, same data except 10-11/ XII/2014; 1♀ 2♂, same data except 7-8/I/2015; 1♂, same data except 12-13/III/2015; 1♀, same data except 6-7/ IV/2015; 1♀, same data except 30/XI-7/XII/2016, funnel light trap; 1♀, same data except 12-18/II/2016; 1♀ 1♂, same data except 22/II-1/III/2017; 1♀, same data except 30/XI-7/XII/2016. Cazorla, C. G. & Campos, R. E. cols.

Stilobezzia (Stilobezzia) glauca Macfie, 1939

Stilobezzia glauca Macfe, 1939: 204 (male; Brazil).

Stilobezzia fuminensis Lane, 1947: 210.

Stilobezzia (Stilobezzia) glauca Wirth & Grogan, 1981:

81; Cazorla, 2016: 188.

Known distribution: Argentina: Buenos Aires: RNIPL, and Misiones. Brazil, Colombia, Mexico, Peru and United States of America.

Material examined: 1♀, marginal forest, 7-8/I/2015, CDC light trap; 1♀ 1♂, same data except 3-4/II/2015. Cazorla, C. G. & Campos, R. E. cols.

Stilobezzia (Stilobezzia) kiefferi Lane 1947

Stilobezzia kiefferi Lane, 1947: 205 (female; Brazil).

Stilobezzia (Stilobezzia) punctipes Wirth, 1953: 79.

Stilobezzia (Stilobezzia) kiefferi Lane & Forattini, 1961: 85; Cazorla, 2016: 188.

Known distribution: Argentina: Buenos Aires: RNIPL. Brazil, Jamaica, Mexico, Panama and United States of America.

Material examined: 1♂, 10/XI/2014, CDC light trap; 2♀, same data except 10/XII/2014. Cazorla, C. G. & Campos, R. E. cols.

Stilobezzia (Stilobezzia) punctulata Lane, 1947

Stilobezzia punctulata Lane, 1947: 204 (female; Brazil).

Stilobezzia (Stilobezzia) punctulata Lane & Forattini, 1958: 220.

Known distribution: Argentina: Buenos Aires: RNIPL (new record), Chaco and Corrientes. Brazil, Mexico, Panama and Peru.

First record in Buenos Aires province.

Material examined: 1♂ 1♀, marginal forest, 10-11/XII/ 2014, CDC light trap. Cazorla, C. G. & Campos, R. E. cols.

Tribu Heteromyiini Wirth, 1962 GenusClinoheleaKieffer

Clinohelea townsendiLane, 1944

Clinohelea townsendi Lane, 1944: 256 (female; Brazil); Spinelli & Duret, 1993: 48.

Clinohelea townesiLane, 1944: 254.

Known distribution: Argentina: Buenos Aires: RNIPL (new record) and Tigre; Corrientes. Brazil, Colombia, Ecuador, French Guiana, Panama, Paraguay and Peru.

Material examined: 3♀, marginal forest, 12-18/II/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

Tribu Palpomyiini Enderlein, 1936

GenusBezziaKieffer

Bezzia(Bezzia) nobilis(Winnertz, 1852)

Ceratopogon nobilis Winnertz, 1852: 79 (female;

Germany).

Bezzia nobilis (Winnertz): Kieffer, 1901: 153 (combination; in key).

Bezzia (Bezzia) nobilis: Wirth, 1983: 673 (review of New World nobilisgroup); Spinelli & Wirth, 1989: 112.

Bezzia acanthodesMacfe, 1940: 192.

Known distribution: Argentina: Buenos Aires: Berisso, Magdalena and RNIPL (new record); Río Negro. Bahamas, Bermuda, Brazil, British Guiana, Canada, Chile, Cuba, Haiti, El Salvador, Honduras, Jamaica, Mexico, United States of America, Uruguay, Virgin Islands and Paleartic Region.

Material examined: 1♂, marginal forest, 10-11/XII/ 2014, CDC light trap; 1♀, same data except, 25/I-1/II/ 2017, funnel light trap; 2♂, same data except, 30/I-7/II/ 2016; 4♀ 1♂, 28/XII- 4/I/2017; 11♂, same data except 22/II-1/III/2017. Cazorla, C. G. & Campos, R. E. cols.

Bezzia punctipennis (Williston, 1896)

Ceratopogon punctipennis Williston, 1896: 278 (female; St. Vicent).

Bezzia punctipennis (Williston): Kieffer, 1906: 58 (combination); Spinelli & Wirth, 1989: 119.

Known distribution: Argentina: Buenos Aires: Berisso, Magdalena and RNIPL (new record). Brazil, Dominica, El Salvador, Haiti, Honduras, Jamaica, Mexico, St. Vincent and United States of America.

Material examined: 1♀, marginal forest, 10-11/XII/ 2014, CDC light trap; 1♀, same data except 12-13/III/ 2015; 1♂, same data except 28/XII-4/I/2017, funnel light trap; 1♀, same data except 30/XI-7/XII/2016, Malaise trap; 1♀, same data except 25/I- 1/II/2017, funnel light trap; 1♀, same data except 22/II-1/III/2017, funnel light trap; 1♀, grassland, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

GenusPalpomyiaMeigen

Palpomyia subaspera(Coquillett, 1901)

Ceratopogon subasperCoquillett, 1901: 606 (female; USA).

Palpomyia subasper Malloch, 1914b: 22 (combination).

Palpomyia subaspera (Coquillett): Grogan & Wirth, 1975: 10 (lectotype designation; redescr.).

Palpomyia essigi Wirth, 1952: 225 (male, female; USA).

Palpomyia maculicrus Ingram & Macfe, 1931: 230 (female; Argentina).

Known distribution: Argentina: Buenos Aires: RNIPL (new record) and San Isidro; Chubut and Río Negro. Canada, Chile, Cuba, Haiti, Mexico, Paraguay and United States of America.

Material examined: 2♀, marginal forest, 12-18/II/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. cols.

 

DISCUSSION

The last study of the family Ceratopogonidae carried out in the Reserva Natural Integral Punta Lara dates back almost thirty years and listed ten known species and seven undetermined species (Spinelli et al., 1989). In the present study, we present an updated list where the number of named species for this protected area of

the province of Buenos Aires is increased to 39. Twenty-three of these species are recorded in the RNIPL for the frst time, while fve species are recorded for the frst time in Buenos Aires province: Dasyhelea mediomunda, Forcipomyia (F.) taragui, Forcipomyia (M.) fuliginosa, Stilobezzia (E.) elegantula and Stilobezzia (S.) punctulata. Most of these species have a Neotropical distribution; therefore, their presence in this area epresents the southernmost limit of their distribution, while Dasyhelea mediomunda presents Andean distribution, so this new record represents the easternmost limit of its distribution. Besides, it is noted that the previously published frst records of Stilobezzia (S. ) glauca and Stilobezzia (S .) kiefferi from Buenos Aires province (Cazorla, 2016) were made based on material from the same series of samples.

Table I. Distribution of Ceratopogonidae species collected in the Reserva Natural ①①: First record in Reserva Natural Integral Punta Lara, ②②: First record in Buenos Aires

Province

Species

cu 09

U

o

XI

XI

o

W

fe

|

a

2

o

_o

4)

CZ2

CZ2

CZ2

es C*2

w

ex

es 03

§0

13

H

Atrichopogon (A.) albinensis

®

Atrichopogon (A.) balseiroi

®

Atrichopogon (A.) comechingon

®

Atrichopogon (A.) homofacies

•

Atrichopogon (A.) talarum

•

Atrichopogon (A.) seudoobfuscatus

®

Atrichopogon (A.) sp. n. A

•

Atrichopogon (L.) delpontei

®

Atrichopogon (P.) domizii

®

Forcipomyia (E.) platensis

®

Forcipomyia (F.) pictoni

®

Forcipomyia (F.) pinamarensis

•

Forcipomyia (F.) poulaineae

®

Forcipomyia (F.) rioplatensis

•

Forcipomyia (F.) taragui

®

Forcipomyia (L.) edmistoni

•

Forcipomyia (M) williamsi

•

Forcipomyia (M) fuliginosa

®

Forcipomyia (S.) leei

®

Forcipomyia (T.) nana

•

Dasyhelea mediomunda

®

Dasyhelea necrophila

®

Dasyhelea serrana

®

Dasyhelea suarezi

®

Culicoides (C) caridei

•

•

Culicoides (H.) debilipalpis

•

•

Culicoides (H.) insignis

•

•

Culicoides (P.) venezuelensis

•

•

Alluaudomyia schnacki

•

Stilobezzia (E.) elegantula

®

Stilobezzia (S.) bicolor

®

Stilobezzia (S.)fiebrigi

•

Stilobezzia (S.) glauca

•

Stilobezzia (S.) kiefferi

•

Stilobezzia (S.) punctulata

®

Clinohelea townsendi

®

Bezzia nobilis

®

Bezzia punctipennis

®

Palpomyia subaspera

®

Integral Punta Lara, by province. province.

 

Forcipomyia and Atrichopogon were the genera with higher number of species, 11 and 9 respectively. Regarding the genera Culicoides, Monohelea Kieffer, Forcipomyia (subgenera Caloforcipomyia, Forcipomyia, Synthyridomyia and Trichohelea) and Palpomyia, some material remains undetermined and is being studied, as they could be new taxa. In addition, an undetermined species of Atrichopogon has resulted to be a new one, which was previously found by Cazorla, Marino and Díaz in Sierra de la Ventana, province of Buenos Aires, and it is currently under description.

We want to express our gratitude to Juan Pablo Carricart, park ranger of the RNIPL and its staff for their kind help and assistance during the collecting. We also thank Dr. Gustavo Spinelli for the loan of the Malaise trap and his willingness to our consults, as well as Lic. Josefina Otero for her assistance with the fgure. This study was supported by grants PIP 0305 and PIP 0399 from Consejo Nacional de Investigaciones Científcas y Técnicas.

Beck, E.C. (1957) Two new species of Culicoides from Florida (Diptera: Heleidae). Florida Entomologist , 40, 103-105.

Borkent, A. (2016) World Species of Biting Midges (Diptera: Ceratopogonidae). Available in: http://www.inhs.uiuc.edu/ research/FLYTREE/CeratopogonidaeCatalog.pdf        [ Links ]

Borkent, A., & Spinelli, G.R. (2007) Neotropical Ceratopogonidae (Diptera: Insecta). Aquatic Biodiversity in Latin America (ABLA) (ed. Adis, J., Arias, J.R., Rueda–Delgado, G., & Wantzen, K.M.), pp 1–198. Pensoft, Sofa–Moscow.         [ Links ]

Borkent, A., & Wirth, W.W. (1997) World species of biting midges (Diptera: Ceratopogonidae). Bulletin of the American Museum of Natural History, 233, 1-257.         [ Links ]

Brèthes, J. (1912) Descripción de un nuevo género y especie nueva de Chironomidae (Dipt.). Anales Museo Nacional de Historia Natural de Buenos Aires, 15, 451-453.         [ Links ]

Brèthes, J. (1914) Description de six Cecidomyidae (Dipt.) de Buenos Aires. Anales Museo Nacional de Historia Natural de Buenos Aires, 26, 151-156.         [ Links ]

Campos, R.E., & Zavortink, T.J. (2010) Description of the larva and pupa and redescription of the adults of Isostomyia paranensis (Brèthes) (Diptera: Culicidae). Zootaxa, 2689, 27-36.         [ Links ]

Cavalieri, F., & Chiossone, I.F. (1972) Notas sobre el género Atrichopogon Kieffer, 1906, de la Argentina (Diptera, Ceratopogonidae). I. Consideraciones generales y descripción de Atrichopogon delponteisp. nov. Revista de la Sociedad Entomológica Argentina, 34, 119-129.         [ Links ]

Cazorla, C.G. (2016) New records of "jejenes" on the subgenus Stilobezzia of Stilobezzia from the Neotropical Region (Diptera: Ceratopogonidae). Revista de la Sociedad Entomológica Argentina, 75(3-4), 186-190.         [ Links ]

Coquillett, D.W. (1901) New Diptera in the U.S. National Museum. Proceedings of the United States National Museum, 23, 593-618.         [ Links ]

Díaz, F., Spinelli, G.R., Donato, M., & Ronderos, M.M. (2014) A taxonomic revision of the Patagonian species of the Dasyhelea mutabilis species group with a phylogenetic analysis of New World species (Diptera: Ceratopogonidae). Journal of Natural History, 48, 2117-2175.         [ Links ]

Díaz, F., Marino, P.I., Ronderos, M.M., & Spinelli, G.R. (2015) Detailed description of larvae and pupae of two Neotropical species of Alluaudomyia Kieffer (Culicomorpha: Ceratopogonidae). Zoologischer Anzeiger, 257, 87-95.

Dinerstein, E., Olson, D.M., Graham, D.J., Webster, A.L., Primm, S.A., Bookbinder, M.P., & Ledec, G. (1995) Una evaluación del estado de conservación de las ecoregiones terrestres de América Latinayel Caribe. WWF and World Bank, Washington D.C.

Donato, M., & Paggi, A. (2005) A new Neotropical species of the genus Metriocnemus van der Wulp (Chironomidae: Orthocladiinae) from Eryngium L. (Apiaceae) phytotelmata. Zootaxa, 1050, 1-14.

Fernández, L.A. (1995) Presencia del subgénero Hydatotrephis MacLeay en la región Neotropical y descripción de Enochrus (Hydatotrephis) bachmanni sp. nov. (Coleoptera: Hydropilidae). Neotropica, 41(105–106), 51-54.

Forattini, O.P., & Rabello E.X. (1956) As formas imaturas de Culicoides guyanensis Floch e Abonnenc, 1942 e de algumas espécies de Stilobezzia (Diptera, Ceratopogonidae). Revista Brasileira de Entomologia, 66, 43-50.

Fox, I. (1946) A review of the species of biting midges of Culicoides from the Caribbean region (Diptera:

Ceratopogonidae). Annals of the Entomological Society of

America, 39, 248-258.

Fox, I. (1952) Six new Neotropical species of Culicoides (Diptera: Ceratopogonidae). Annals of the Entomological Society of America, 45, 364-368.

Fox, I., & Hoffman W.A. (1944) New Neotropical biting sand fies of the genus Culicoides(Diptera: Ceratopogonidae). Puerto Rico Journal of Public Health and Tropical Medicine, 20, 108-111

García, J.J., Campos, R.E., & Maciá, A. (1994) Prospección de enemigos naturales de Culicidae (Diptera) de la selva marginal de Punta Lara (Prov. de Buenos Aires, República Argentina). Revista de La Academia de Ciencias Exactas y Naturales de Colombia, 19, 209-215.

García, J.J., Campos, R.E., & Maciá, A. (1995) Observaciones ecológicas sobre Mansonia indubitans y Ma. titillans (Diptera: Culicidae) y sus enemigos naturales en Punta Lara, Argentina. Revista de la Sociedad Entomológica Argentina, 54(1-4), 43-50.

Goetghebuer, M. (1933) Ceratopogonidae et Chironomidae du Congo Belge. Revue de Zoologie et de Botanique Africaines, 24, 129-151.

Grogan, W.L., & Wirth, W.W. (1975) A revision of the genus Palpomyia Meigen of the northeastern North America (Diptera: Ceratopogonidae). University of Maryland Agricultural Experiment Station Miscellaneous Publications, 875.

Herrera, R., & Torres Robles, S. (2012) Comunidades vegetales de la Reserva Natural Punta Lara. Inventario de los vertebrados de la Reserva Natural de Punta Lara, provincia de Buenos Aires, Argentina(ed. Roesler, I., & Agostini, M.G.) Temas de Naturaleza y Conservación, Monografía de Aves Argentinas Nº 8, 8 pp. Buenos Aires, Argentina.

Ingram, A., & Macfe, J.W.S. (1931) Ceratopogonidae. Diptera of Patagonia and South Chile, based mainly on material in the British Museum (Natural History), part II, Fasc. 4, Nematocera(ed. Edwards, F.W), pp. 155-232. The Trustees, London

Johannsen, O.A. (1907) Some new species of Kansas Chironomidae. Some results of desultory collecting of insects in Kansas and Colorado(ed. Tucker, E.S.). Bulletin of the University of Kansas, Science Bulletin.

Johannsen, O.A. (1934) New species of North American Ceratopogonidae and Chironomidae. Journal of the New York Entomological Society, 42, 343-352.

Johannsen, O.A. (1943) Two new species of American Ceratopogonidae (Diptera). Annals of the Entomological Society of America, 36, 761-762.

Kieffer, J.J. (1901) Synopse des representants européens du group Ceratopogonavec description de quelques especies nouvelles. Bulletin de la Société d'Historie Naturelle de Metz, 9, 143-165.

Kieffer, J.J. (1906) Diptera. Fam. Chironomidae. Genera Insectorum, Fasc. 42(ed. Wytsman, P.), pp. 1–78.

Kieffer, J.J. (1917) Chironomides d’Amérique conservés au Musée National Hongrois de Budapest. Annales historico–naturales Musei nationalis hungarici, 15, 292-364.

Knab, F. (1914) Ceratopogoninae sucking the blood of caterpillars. Proceedings of the Entomological Society of Washington, 16, 63-66.

Lane, J. (1944) As Espécies Neotropicas do Genero Clinohelea Kieffer, 1917 (Diptera, Ceratopogonidae). Revista de Entomologia.15, 249-261.

Lane, J. (1945) Redescrição de Ceratopogonideos Neotropicos (Diptera: Ceratopogonidae). Revista de Entomologia, 16, 357-372.

Lane, J. (1947) A biologia e taxonomia de algumas espécies dos grupos Forcipomyia e Culicoides (Diptera, Ceratopogonidae) (Heleidae). Arquivos da Faculdade de HigieneeSaúde Pública da Universidade de São Paulo, 1, 159-170.

Lane, J., & Forattini, O.P. (1956) Neotropical StilobezziaKieffer, 1911 I. Nine new Panamanian species (Diptera, Nematocera, Ceratopogonidae). Revista Brasileira de Malariologia, 8, 207-226.

Lane, J., & Forattini, O.P. (1958) Neotropical Stilobezzia II. Fourteen new species, chiefy from Panama (Diptera: Ceratopogonidae). Revista Brasileira de Entomologia, 8, 203-224.

Lane, J., & Forattini, O.P. (1961) Neotropical Stilobezzia, 1911 III. Key for the adults of this genus and description of one new species (Diptera, Ceratopogonidae). Revista Brasileira de Entomologia, 10, 83-94.

Lane, J., & Ortíz, I. (1950) Sinonimia en Culicoides (Diptera: Ceratopogonidae). Revista de SanidadyAsistencia Social, 15, 460.

Lutz, A. (1913) Contribuição para o estudo das Ceratopogoninas hematofagas do Brazil. Memórias do Instituto Oswaldo Cruz, 5, 45-73.

Maciá, A., García, J.J., & Campos, R.E. (1995) Bionomía de Aedes albifasciatus y Aedes crinifer (Diptera: Culicidae) y sus enemigos naturales en Punta Lara, provincia de Buenos Aires, República Argentina. Neotropica, 41, 43-50.

Maciá, A., García, J.J., & Campos, R.E. (1997) Variación estacional de tres especies de Culex (Diptera: Culicidae) y sus parásitos y patógenos en Punta Lara, provincia de Buenos Aires. Revista de Biología Tropical, 44(4), 267-275.

Macfe, J.W.S. (1938) Notes on Ceratopogonidae (Diptera). Proceedings of the Entomological Society of Washington (B), 7, 157-166.

Macfe, J.W.S. (1939) A report on a collection of Brazilian Ceratopogonidae (Dipt.). Revista de Entomología, 10, 137-219.

Macfe, J.W.S. (1940) Ceratopogonidae (Diptera) from British Guiana and Trinidad. Part 2. Proceedings of the Royal Entomological Society of London, 9, 187-195.

Malloch, J.R. (1914a) Synopsis of the genus Probezzia, with description of a new species. Proceedings of the Biological Society of Washington, 27, 137-140.

Malloch, J.R. (1914b) Notes on North American Diptera, with description of a new species in the collection of the Illinois State Laboratory of Natural History. Bulletin of the Illinois State Laboratory of Natural History, 10, 213-243.

Malloch, J.R. (1915) The Chironomidae, or midges, of Illinois, with particular reference to the species occurring in the Illinois river. Bulletin of the Illinois State Laboratory of Natural History, 10, 275-543.

Marino, P.I., & Spinelli, G.R. (1999a) The species groups of the subgenus Forcipomyia (Forcipomyia) in the Neotropics with a description of a new species of the genualis group (Diptera: Ceratopogonidae). Transactions of the American Entomological Society, 125(4), 445-452.

Marino, P.I., & Spinelli, G.R. (1999b) The subgenus Forcipomyia (Metaforcipomyia) in Argentina (Diptera: Ceratopogonidae). Iheringia, série Zoologia, 86, 3-8.

Marino, P.I., & Spinelli, G.R. (2002) A revision of the Forcipomyia squamitibia group in the Neotropics with the description of three new species (Diptera: Ceratopogonidae). Insect Science and its Application, 22(4), 307-319.

Marino, P.I., Spinelli, G.R., & Cazorla, C.G. (2002) Type–specimens of Ceratopogonidae (Insecta: Diptera) in the collection of the Museo de La Plata, Argentina. Publicación Técnica yDidáctica, Facultad de Ciencias Naturales y Museo, UNLP, 42, 1-37.

Marino, P.I., Cazorla, C.G., & Díaz, M.F. (2011) Los Ceratopogonidae (Diptera: Nematocera) del Sistema Serrano de Ventania, Buenos Aires, Argentina. Revista de la Sociedad Entomológica Argentina, 70(3-4), 197-205.

Marino, P.I., Spinelli, G.R., Ferreira–Keppler, R., & Ronderos, M.M. (2017) Description of fourth instar larva and pupa of Atrichopogon delpontei Cavalieri and Chiossone (Diptera: Ceratopogonidae) from Brazilian Amazonia. Anais da Academia Brasileira de Ciências, 89(3), 2081-2094.

Mauad, M., Siri, A., & Donato, M. (2013) New species of Pseudosmittia Edwards, 1932 and new records of Allocladius Kieffer, 1913 (Diptera: Chironomidae, Orthocladiinae) from South America. Zootaxa, 3694, 445-460.

Meigen, J.W. (1818) Systematische Beschreibung der bekannten europäischen zweifügeligen Insekten. Vol. 1. Aachen.

Minaya, G. (1978) Dasyhelea mediomunda, sp. n. (Diptera: Ceratopogonidae) de la costa central del Perú. Revista Peruana de Entomología, 21, 79-81.

Morrone, J.J. (2014) Biogeographical regionalization of the Neotropical region. Zootaxa, 3782, 1-110.

New, T.R. (2012) Introduction to insect conservation, an emerging discipline. Insect conservation: past, present and prospects (ed. New, T.R.), pp. 1-17. Dordrecht, Springer, USA.

Ortíz, I., & Mirsa, M. (1950) Estudios en Culicoides (Diptera: Ceratopogonidae). Sobre una nueva especie del grupo haematopotus: Culicoides venezuelensis n. sp. Archivos Venezolanos de Patologia TropicalyParasitologia Medica, 2, 137-144.

Philippi, R.A. (1865) Aufzählung der chilenischen Dipteren. Abhandlungen der Zoologisch-Botanischen Gesellschaft in Wien, 15, 595-782.

Rodrigues Capítulo, A. (1988) Micrathyria ringueletispec. nov. from Argentina and its larva (Anisoptera: Libellulidae). Odonatologica, 17(4), 409-418.

Rodrigues Capítulo, A. (1996) A Description of the last instar larva of Tauriphila risi (Anisoptera: Libellulidae). Odonatologica, 25, 391-395.

Rodrigues Capítulo, A., & Muzón, J. (1990) The larval instars of Orthemis nodiplaga Karsch, 1891, from Argentina (Anisoptera: Libellulidae). Odonatologica, 19, 283-291.

Ronderos, M.M., & Spinelli, G.R. (1990) Further notes on Culicoides Latreille 1809 from Argentina (Insecta, Diptera, Ceratopogonidae). Iheringia, Série Zoología, 70, 81-88.

Ronderos, M.M., & Spinelli, G.R. (1995) Redescripcion de Culicoides lahillei y descripcion de Culicoides ichesi, n. sp. de la Argentina, Paraguay y Uruguay (Diptera: Ceratopogonidae). Neotropica, 41(105-106), 77-81.

Ronderos, M.M., Marino, P.I, Díaz, F., & Estévez, A.L. (2011) Biting midges (Diptera: Ceratopogonidae) from Martín García Island, Argentina. Revista de Biología Tropical, 59(3), 1183-1194.

Ronderos, M.M., Marino, P.I., & Díaz, F. (2017) First description of the pupa and male of the Neotropical predatory midge Pellucidomyia oliveirai (Lane) (Diptera: Ceratopogonidae). Annales de Limnologie-International Journal of Limnologie, 53, 57–65.

Schnack, J.A, Spinelli, G.R, Estévez, A.L., & Domizi, E.A. (1986) Aspectos de la ecología reproductiva de poblaciones bonaerenses de Belostoma elegans(Mayr) y B. micantulum (Stal) (Hemiptera, Belostomatidae). Revista de la Asociación de Ciencias Naturales del Litoral, 17, 31-37.

Schnack, J.A, Domizi, E.A, Estévez, A.L., & Spinelli, G.R. (1989) Ecología del comportamiento reproductivo de Belostomatinae. I. Sistemas de apareamiento en poblaciones del área platense de Belostoma oxyurum, B. elegans y B. micantulum (Hemiptera, Belostomatidae). Revista de la Sociedad Entomológica Argentina, 45, 85-94.

Siri, A., Marti, G., & Lopez Lastra, C. (2008) Prevalence of Harpellales from Chironomidae larvae in phytotelmata from Punta Lara Forest, Argentina. Mycologia, 100(3), 381-386.

Spinelli, G.R. (1982) Cuatro especies nuevas del género Atrichopogon Kieffer (Diptera: Ceratopogonidae) en la República Argentina. Revista de la Sociedad Entomológica Argentina, 41(1-4), 201-210.

Spinelli, G.R. (1983a) Notas sobre Ceratopogonidae (Diptera: Nematocera) de la República Argentina. II. Nuevos aportes al conocimiento del género Forcipomyia Meigen. Neotropica, 29, 121-129.

Spinelli, G.R. (1983b) Notas sobre Ceratopogonidae (Diptera: Nematocera) de la República Argentina. I. Nueva especie del género AlluaudomyiaKiffer, redescripción de Dasyhelea penthesileae Macfe, y nuevas citas para el género StilobezziaKieffer. Limnobios, 2(6), 403-411.

Spinelli, G.R., & Balseiro, E.G. (1982) Observaciones relativas a la actividad nocturna de Ceratopogonidae (Diptera: Nematocera) en la zona de Los Talas (Provincia de Buenos Aires), mediante el empleo de una trampa de luz.Revista de la Sociedad Entomológica Argentina, 41(1-4), 225-232.

Spinelli, G.R., & Duret, J.P. (1993) Las especies neotropicales de Clinohelea (Diptera: Ceratopogonidae). Graellsia, 49, 39-50.

Spinelli, G.R., & Marino, P.I. (1998) First records for Argentina of three species of Forcipomyia(Diptera: Ceratopogonidae). Revista de la Sociedad Entomológica Argentina, 57, 39-40.

Spinelli, G.R., & Rodríguez, E.A. (1999) A new species of Dasyhelea from Argentina described as adult, pupae and fourth instar larvae (Diptera: Ceratopogonidae). Neotropica, 45, 59-62.

Spinelli, G.R., & Ronderos, M.M. (1987) Notas sobre Ceratopogonidae (Diptera: Nematocera) de la República Argentina. V. Nuevos aportes al conocimiento del género DasyheleaKieffer. Neotropica, 33, 11-17.

Spinelli, G.R., & Wirth, W.W. (1986) Clave la identifcación de las especies del género CulicoidesLatreille presentes al sur de la cuenca Amazónica, nuevas citas y notas sinonímicas (Diptera: Ceratopogonidae). Revista de la Sociedad Entomológica Argentina, 44, 49-73.

Spinelli, G.R., & Wirth, W.W. (1989) Las especies neotropicales del género Bezzia (Diptera: Ceratopogonidae). II. Los grupos nobilis y punctipennis. Revista de la Sociedad Entomológica Argentina, 45, 109-129.

Spinelli, G.R., Ronderos, M.M., & Balseiro, E.G. (1989) Análisis de la actividad diaria de dípteros nematoceros en Punta Lara (Pdo. Ensenada, Prov. de Buenos Aires) II. Ceratopogonidae con la descripción de una especie nueva en el género Atrichopogon. Limnobios, 2(10), 733-737.

Spinelli, G.R., Marino, P.I., & Ronderos, M.M. (2005) The fourth instar larva and pupa of the Neotropical biting midge Forcipomyia (Forcipomyia) rioplatensis Marino and Spinelli (Diptera: Ceratopogonidae). Proceedings of the Entomological Society of Washington, 107, 108-114.

Spinelli, G.R., Marino, P.I., & Posadas, P. (2006) The Patagonian species of the genus Atrichopogon Kieffer, with a biogeographic analysis based on Forcipomyiinae (Diptera: Ceratopogonidae). Insect Systematics&Evolution, 37, 301-324.

Spinelli, G.R., Marino, P.I., & Gaddi, A.L. (2010) New records of biting midges from Argentina and Chile (Diptera: Ceratopogonidae). Revista de la Sociedad Entomológica Argentina, 69(1-2), 127-132.

Spinelli, G.R, Marino P.I., & Huerta, H. (2015) Revision of the Neotropical species of the subgenus Atrichopogon (Psilokempia) (Diptera: Ceratopogonidae). Zootaxa, 4003(1), 1-64.

UNESCO (2017) Man and the Biosphere Programme. Available in: http://www.unesco.org/mab/

Vieira, L.C., Oliveira, N.G., Brewster, C.C., & Gayubo, S.F. (2013) Faunistic inventory of Spheciformes wasps at three protected areas in Portugal. Journal of Insect Science, 13, 113.

Von Ellenrieder, N. (2000) Species composition and temporal variation of odonate assemblages in the subtropical–pampasic ecotone, Buenos Aires, Argentina. Odonatologica, 29(1), 17-30.

Von Ellenrieder, N., & Fernandez, L. (2000) Aquatic Coleoptera in the Subtropical–Pampasic Ecotone (Argentina, Buenos Aires): Species composition and temporal changes. Coleopterists Bulletin, 54(1), 23-35.

Von Ellenrieder, N., & Perez Goodwyn, P. (2000) Species composition and temporal variation of aquatic Heteroptera (Insecta) in the subtropical–Pampasic ecotone in Argentina. Revista Brasileira de Entomologia, 44, 43-50.

Williston, S.W. (1896) On the Diptera of St. Vincent (West Indies). Transactions of the Entomological Society of London, 1896, 253-446.

Winnertz, J. (1852) Beitrage zur Kenntnis der Gattung Ceratopogon. Linnaea Entomologica, 6, 1-180.

Wirth, W.W. (1952) The Heleidae of California. University of California Publications on Entomology, 9, 95-266.

Wirth, W.W. (1953) Biting midges of the heleid genus Stilobezzia in North America. Proceedings of the United States National Museum, 103, 57-58.

Wirth, W.W. (1955) The biting midge genera Psychophaenaand Tetraphoraof Philippi 1865. Revista Chilena de Entomología, 4, 233-235.

Wirth, W.W. (1956) The heleid midges involved in the pollination of rubber trees in America (Diptera, Heleidae). Proceedings of the Entomological Society of Washington, 58, 241-250.

Wirth, W.W. (1972) The Neotropical Forcipomyia (Microhelea) species related to the caterpillar parasite F. fuliginosa (Diptera: Ceratopogonidae). Annals of the Entomological Society of America, 65, 564-577.

Wirth, W.W. (1974) Family Ceratopogonidae. A catalogue of the Diptera of the Americas South of the United States, fasc. 14. Museu de Zoologia, Universidade de São Paulo, Brazil.

Wirth, W.W. (1983) A review of the American predaceous midges of the Bezzia nobilis group (Diptera: Ceratopogonidae). Proceedings of the Entomological Society of Washington, 85, 670-685.

Wirth, W.W., & Cavalieri, F. (1975) Sobre la identidad de Euprojoannisia platensis Brèthes 1914 (Diptera: Ceratopogonidae). Neotropica, 21(66), 125-126.

Wirth, W.W., & Grogan, W.L. (1981) Natural History of Plummers Island, Maryland XXV. Biting midges (Diptera: Ceratopogonidae). 3. The species of the tribe Stilobezziini. Bulletin of the Biological Society of Washington, 5, 1-102.

Wirth, W.W., & Ratanaworabhan, N.C. (1978) Studies on the genus Forcipomyia. V. Key to subgenera and description of a new subgenus related to EuprojoannisiaBrèthes (Diptera: Ceratopogonidae). Proceedings of the Entomological Society of Washington, 80, 493-507.

Wirth, W.W., & Spinelli, G.R. (1993) The North American species of the Forcipomyia(Lepidohelea) bicolorsubgroup (Diptera: Ceratopogonidae). Proceedings of the Entomological Society of Washington, 95, 611-634.

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License